Coupling Bifunctional Nanozyme-Mediated Catalytic Signal Amplification and Label-Free SERS with Immunoassays for Ultrasensitive Detection of Pathogens in Milk Samples

• Published in: Analytical chemistry (Washington) 2023-04, Vol.95 (15), p.6417-6424
• Main Authors: Li, Zhihao, Hu, Jiao, Zhan, Yifang, Shao, Zhiyong, Gao, Mengyue, Yao, Qi, Li, Zheng, Sun, Shaowen, Wang, Lihua
• Format: Article
• Language: English
• Subjects: Amplification; Analytical chemistry; Animals; Biosensing Techniques; Biosensors; Chemistry; Coupling (molecular); Food Safety; Foodborne Diseases; Foodborne pathogens; Gold; Gold - chemistry; Humans; Immunoassay; Immunoassays; Metal Nanoparticles - chemistry; Milk; Pathogens; Platinum; Raman spectra; Salmonella Typhi; Sensitivity; Spectrum Analysis, Raman; Substrates
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.3c00251

Rapid and sensitive detection of foodborne bacteria is of great significance in guaranteeing food safety and preventing foodborne diseases. A bifunctional Au@Pt core–shell nanozyme with excellent catalytic properties and high surface-enhanced Raman scattering (SERS) activity was developed for the highly sensitive detection of Salmonella typhimurium based on a label-free SERS strategy. The ultrathin Pt shell (about 1 nm) can catalyze Raman-inactive molecules into Raman-active reporters, greatly amplifying the amount of signal molecules. Moreover, the Au core serves as an active SERS substrate to enhance the signal of reporter molecules, further significantly improving the detection sensitivity. Benefiting from the excellent properties, such a bifunctional Au@Pt nanozyme was integrated with a magnetic immunoassay to construct a label-free SERS platform for the highly sensitive detection of S. typhi with a low detection limit of 10 CFU mL–1. Also, the Au@Pt-based SERS platform exhibited excellent selectivity and was successfully utilized for the detection of S. typhi in milk samples by a portable Raman spectrometer. Our demonstration of the bifunctional nanozyme-based SERS strategy provides an efficient pathway to improve the sensitivity of label-free SERS detection of pathogens and holds great promise in food safety, environmental analysis, and other biosensing fields.