Antibiotic Susceptibility, Biofilm-Forming Ability, and Prevalence of Extended-Spectrum Beta-Lactamase (ESBL)- and Biofilm-Associated Genes Among Klebsiella pneumoniae Isolates from Hospitalized Patients in Northwest of Iran

Klebsiella pneumoniae is an opportunistic bacterium, which is globally recognized for its high prevalence and antimicrobial resistance (AMR). Biofilm-forming capability, susceptibility testing, and phenotypic confirmatory test for extended-spectrum beta-lactamase (ESBL)-producing isolate recognition...

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Bibliographic Details
Published in:Current microbiology 2023-05, Vol.80 (5), p.175-175, Article 175
Main Authors: Mirzaei, Bahman, Ebrahimi, Aida, Keshavarzi, Shima, Hydarzadeh, Siamak, Badmasti, Farzad, Dadar, Maryam, Moradi, Narges
Format: Article
Language:English
Subjects:
Ampicillin
Anti-Bacterial Agents - pharmacology
Antimicrobial resistance
beta-Lactamases - genetics
Biofilms
Biomedical and Life Sciences
Biotechnology
Colistin
Drug resistance
Escherichia coli - genetics
Genes
Humans
Iran - epidemiology
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Life Sciences
LuxS protein
Microbial Sensitivity Tests
Microbiology
Prevalence
β Lactamase
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Summary:Klebsiella pneumoniae is an opportunistic bacterium, which is globally recognized for its high prevalence and antimicrobial resistance (AMR). Biofilm-forming capability, susceptibility testing, and phenotypic confirmatory test for extended-spectrum beta-lactamase (ESBL)-producing isolate recognition of 104 K . pneumoniae isolates were performed according to the Clinical Laboratory Standard Institute (CLSI) guidelines. The prevalence of ESBL-associated genes bla-VIM , bla-NDM , and bla-OXA-48 , as well as biofilm-associated genes luxS , fimH1 , wza , and mrkD , was determined by multiplex PCR. The highest resistance rate was against ampicillin (100.0%). Among the 104 K . pneumoniae isolates, 52 (50.0%) and 31 (29.8%) isolates were determined as multi- and extensively drug resistant (MDR, XDR), respectively. Moreover, 21 (40.4%) isolates were determined as ESBL producing. Among 50 biofilm-producing K. pneumoniae isolates, 7 (14.0%), 15 (30.0%), and 28 (56.0%) isolates exhibited high, moderate, and weak levels of biofilm formation, respectively. A number of 41 (78.8%) isolates were susceptible to colistin, and 10 (19.2%) were resistant. AMR was significantly higher ( P  
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-023-03247-7