Antimicrobial, Antibiofilm Activities and Synergic Effect of Triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene Isolated from Combretum leprosum Leaves Against Staphylococcus Strains

• Published in: Current microbiology 2023-05, Vol.80 (5), p.176-176, Article 176
• Main Authors: Teixeira, Edson Holanda, Andrade, Alexandre Lopes, Pereira, Rafael, Farias, Livia Pontes, Monteiro, Gabrieli Sobral, Marinho, Marcia Machado, Marinho, Emmanuel Silva, Santos, Hélcio Silva, de Vasconcelos, Mayron Alves
• Format: Article
• Language: English
• Subjects: Ampicillin; Ampicillin - pharmacology; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bactericidal activity; Biofilms; Biomedical and Life Sciences; Biotechnology; Cell morphology; Combretum - chemistry; Cytology; Drug resistance; Enumeration; Evaluation; Gentian violet; Life Sciences; Methicillin; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Microbiology; Microorganisms; Minimum inhibitory concentration; Molecular docking; Molecular Docking Simulation; Penicillin; Penicillin-binding protein; Plant Extracts - pharmacology; Proteins; Public health; Scanning electron microscopy; Staphylococcus; Staphylococcus aureus; Staphylococcus epidermidis; Strains (organisms); Synergistic effect; Triterpenes - pharmacology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-023-03284-2

Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene (CLF1) against Staphylococcus aureus and Staphylococcus epidermidis strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for S. epidermidis strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of S. aureus ATCC 700698 biofilms (a methicillin-resistant S. aureus strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against Staphylococcus biofilms.