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Antimicrobial, Antibiofilm Activities and Synergic Effect of Triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene Isolated from Combretum leprosum Leaves Against Staphylococcus Strains
Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of...
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| Published in: | Current microbiology 2023-05, Vol.80 (5), p.176-176, Article 176 |
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| container_end_page | 176 |
| container_issue | 5 |
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| container_title | Current microbiology |
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| creator | Teixeira, Edson Holanda Andrade, Alexandre Lopes Pereira, Rafael Farias, Livia Pontes Monteiro, Gabrieli Sobral Marinho, Marcia Machado Marinho, Emmanuel Silva Santos, Hélcio Silva de Vasconcelos, Mayron Alves |
| description | Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene (CLF1) against
Staphylococcus aureus
and
Staphylococcus epidermidis
strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for
S. epidermidis
strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of
S. aureus
ATCC 700698 biofilms (a methicillin-resistant
S. aureus
strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against
Staphylococcus
biofilms. |
| doi_str_mv | 10.1007/s00284-023-03284-2 |
| format | article |
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Staphylococcus aureus
and
Staphylococcus epidermidis
strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for
S. epidermidis
strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of
S. aureus
ATCC 700698 biofilms (a methicillin-resistant
S. aureus
strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against
Staphylococcus
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Staphylococcus aureus
and
Staphylococcus epidermidis
strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for
S. epidermidis
strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of
S. aureus
ATCC 700698 biofilms (a methicillin-resistant
S. aureus
strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against
Staphylococcus
biofilms.</description><subject>Ampicillin</subject><subject>Ampicillin - pharmacology</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bactericidal activity</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell morphology</subject><subject>Combretum - chemistry</subject><subject>Cytology</subject><subject>Drug resistance</subject><subject>Enumeration</subject><subject>Evaluation</subject><subject>Gentian violet</subject><subject>Life Sciences</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Minimum inhibitory concentration</subject><subject>Molecular docking</subject><subject>Molecular Docking Simulation</subject><subject>Penicillin</subject><subject>Penicillin-binding protein</subject><subject>Plant Extracts - 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Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, Edson Holanda</au><au>Andrade, Alexandre Lopes</au><au>Pereira, Rafael</au><au>Farias, Livia Pontes</au><au>Monteiro, Gabrieli Sobral</au><au>Marinho, Marcia Machado</au><au>Marinho, Emmanuel Silva</au><au>Santos, Hélcio Silva</au><au>de Vasconcelos, Mayron Alves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial, Antibiofilm Activities and Synergic Effect of Triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene Isolated from Combretum leprosum Leaves Against Staphylococcus Strains</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2023-05-01</date><risdate>2023</risdate><volume>80</volume><issue>5</issue><spage>176</spage><epage>176</epage><pages>176-176</pages><artnum>176</artnum><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene (CLF1) against
Staphylococcus aureus
and
Staphylococcus epidermidis
strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for
S. epidermidis
strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of
S. aureus
ATCC 700698 biofilms (a methicillin-resistant
S. aureus
strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against
Staphylococcus
biofilms.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37029832</pmid><doi>10.1007/s00284-023-03284-2</doi><tpages>1</tpages></addata></record> |
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| ispartof | Current microbiology, 2023-05, Vol.80 (5), p.176-176, Article 176 |
| issn | 0343-8651 1432-0991 |
| language | eng |
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| source | Springer Nature |
| subjects | Ampicillin Ampicillin - pharmacology Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Antimicrobial resistance Bactericidal activity Biofilms Biomedical and Life Sciences Biotechnology Cell morphology Combretum - chemistry Cytology Drug resistance Enumeration Evaluation Gentian violet Life Sciences Methicillin Methicillin-Resistant Staphylococcus aureus Microbial Sensitivity Tests Microbiology Microorganisms Minimum inhibitory concentration Molecular docking Molecular Docking Simulation Penicillin Penicillin-binding protein Plant Extracts - pharmacology Proteins Public health Scanning electron microscopy Staphylococcus Staphylococcus aureus Staphylococcus epidermidis Strains (organisms) Synergistic effect Triterpenes - pharmacology |
| title | Antimicrobial, Antibiofilm Activities and Synergic Effect of Triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene Isolated from Combretum leprosum Leaves Against Staphylococcus Strains |
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