Spatial metabolic heterogeneity of oligodendrogliomas at single-cell resolution

Oligodendrogliomas are a type of rare and incurable gliomas whose metabolic profiles have yet to be fully examined. The present study examined the spatial differences in metabolic landscapes underlying oligodendrogliomas and should provide unique insights into the metabolic characteristics of these...

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Bibliographic Details
Published in:Brain tumor pathology 2023-04, Vol.40 (2), p.101-108
Main Authors: Batchu, Sai, Diaz, Michael Joseph, Kleinberg, Giona, Lucke-Wold, Brandon
Format: Article
Language:English
Subjects:
Amino acids
Biosynthesis
Brain cancer
Brain Neoplasms - pathology
Cancer Research
Cell cycle
Chemotherapy
Chromosome Deletion
Chromosomes
Chromosomes, Human, Pair 1 - genetics
Chromosomes, Human, Pair 19
Gene expression
Glioma - genetics
Humans
Isocitrate Dehydrogenase - genetics
Isocitrate Dehydrogenase - metabolism
Medicine
Medicine & Public Health
Metabolism
Metabolites
Mortality
Mutation
Neurology
Neurosurgery
Oligodendroglioma - genetics
Oligodendroglioma - pathology
Oncology
Original Article
Pathology
Patients
Tumors
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Summary:Oligodendrogliomas are a type of rare and incurable gliomas whose metabolic profiles have yet to be fully examined. The present study examined the spatial differences in metabolic landscapes underlying oligodendrogliomas and should provide unique insights into the metabolic characteristics of these uncommon tumors. Single-cell RNA-sequencing expression profiles from 4044 oligodendroglioma cells derived from tumors resected from four locations frontal, temporal, parietal, and frontotemporoinsular) and in which 1p/19q co-deletion and IDH1 or IDH2 mutations were confirmed were computationally analyzed through a robust workflow to elucidate relative differences in metabolic pathway activities among the different locations. Dimensionality reduction using metabolic expression profiles exhibited clustering corresponding to each location subgroup. From the 80 metabolic pathways examined, over 70 pathways had significantly different activity scores between location subgroups. Further analysis of metabolic heterogeneity suggests that mitochondrial oxidative phosphorylation accounts for considerable metabolic variation within the same locations. Steroid and fatty acid metabolism pathways were also found to be major contributors to heterogeneity. Oligodendrogliomas display distinct spatial metabolic differences in addition to intra-location metabolic heterogeneity.
ISSN:1433-7398
1861-387X
DOI:10.1007/s10014-023-00455-8