Profile of Human Milk Phospholipids at Different Lactation Stages with UPLC/Q-TOF-MS: Characterization, Distribution, and Differences

Human milk phospholipids are important for the regular growth and development of infants. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was employed to qualitatively and quantitatively analyze 277 phospholipid molecular species in 112 human...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2023-04, Vol.71 (16), p.6326-6337
Main Authors: Ding, Desheng, He, Xiaoling, Agarry, Israel Emiezi, Wang, Yuankai, Zhou, Fenglan, Li, Yunchang, Kan, Jianquan, Cai, Tian, Chen, Kewei
Format: Article
Language:English
Subjects:
Bioactive Constituents, Metabolites, and Functions
Chromatography, High Pressure Liquid
Female
Humans
Lactation
Lecithins
Milk, Human - chemistry
Phosphatidylcholines - analysis
Phosphatidylethanolamines
Phosphatidylinositols - analysis
Phosphatidylserines
Phospholipids - analysis
Sphingomyelins
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human milk phospholipids are important for the regular growth and development of infants. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was employed to qualitatively and quantitatively analyze 277 phospholipid molecular species in 112 human milk samples to obtain a detailed profile of human milk phospholipids along the lactation stage. MS/MS fragmentation patterns of sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine were characterized in detail. Phosphatidylcholine is the most dominant group, followed by sphingomyelin. PC­(18:0/18:2), SM­(d18:1/24:1), PE­(18:0/18:0), PS­(18:0/20:4), and PI­(18:0/18:2) showed the highest average concentration among all of the phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol molecular species, respectively. The fatty acids attached to the phospholipid molecules were mainly palmitic, stearic, oleic, and linoleic acids, and the plasmalogens decreased along the lactation stage. The increase of sphingomyelins and phosphatidylethanolamines and the decrease of phosphatidylcholines are the key changes from colostrum to transitional milk; the increase of lysophosphatidylcholines and lysophosphatidylethanolamines and the continuous decrease of phosphatidylcholines are the vital changes from transitional milk to mature milk.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.2c07512