Complete genome sequence of daphne virus 1, a novel cytorhabdovirus infecting Daphne odora

• Published in: Archives of virology 2023-05, Vol.168 (5), p.141-141, Article 141
• Main Authors: Belete, Mesele Tilahun, Kim, Se Eun, Igori, Davaajargal, Ahn, Jun Ki, Seo, Han Kyu, Park, Yang Chan, Moon, Jae Sun
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; Cloning; Daphne - genetics; Genes; Genome, Viral; Genomes; Genomics; Infectious Diseases; Medical Microbiology; New species; Next-generation sequencing; Nucleotide sequence; Nucleotides; Open Reading Frames; Phylogeny; Plant Diseases; Plant viruses; Proteins; Rhabdoviridae - genetics; RNA polymerase; RNA, Viral - genetics; Viral Proteins - genetics; Virology; Viruses
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-023-05734-5

A novel cytorhabdovirus was identified in Daphne odora in South Korea using high-throughput sequencing. The virus, tentatively named “daphne virus 1” (DV1), has a full-length genome sequence of 13,206 nucleotides with a genome organization comparable to that of unsegmented plant rhabdoviruses and contains seven antisense putative genes in the order 3′-leader-N-P′-P-P3-M-G-L-5′-trailer. The coding region of the genome is flanked by a 3′ leader and a 5′ trailer sequence, 261 and 151 nucleotides long, respectively. The DV1 genome shares 33.74%–57.44% nucleotide sequence identity with other cytorhabdoviruses. The DV1-encoded proteins share the highest amino acid sequence identity with homologues from Asclepias syriaca virus 1. Phylogenetic analysis showed that DV1 clustered with representative cytorhabdoviruses. We propose classifying DV1 in a new species within the genus Cytorhabdoviru s, family Rhabdoviridae .