Oxidation and reduction analysis of therapeutic recombinant human interleukin-15 by HPLC and LC-MS

• Published in: Applied microbiology and biotechnology 2023-05, Vol.107 (10), p.3217-3227
• Main Authors: Wang, Yang, Chen, Huanhuan, Zhao, Meiqi, Feng, Lei, Liu, Zexin, Zeng, Qiongya, Shi, Wenqiang, Zhu, Wen, Song, Luyao, Zhu, Jianwei, Lu, Huili
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; By products; Byproducts; Chemical properties; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid; Cytokines; Fractions; Health aspects; High performance liquid chromatography; Humans; Hydrogen atoms; Immunotherapy; Impurities; Interleukin 15; Interleukin-2 - chemistry; Life Sciences; Liquid chromatography; Lymphocytes; Lymphocytes T; Manufacturing; Microbial Genetics and Genomics; Microbiology; Natural killer cells; Oxidation; Oxidation-Reduction; Oxidation-reduction reaction; Oxygen; Peptide mapping; Production processes; Protein research; Quality control; Recombinant proteins; Recombinant Proteins - metabolism; Reduction; Streamlining; Tandem Mass Spectrometry
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-023-12508-1

Being an important immune stimulant of T lymphocytes and NK cells, the recombinant human interleukin-15 (rhIL-15) has been extensively researched in tumor immunotherapy or as a vaccine adjuvant. However, the rhIL-15 manufacturing level lags far behind its growing clinical demand due to the lack of efficient and exact analysis methodologies to characterize the trace by-products, typically redox and deamidation. In order to improve the production and quality control of rhIL-15, here we developed an expanded resolution reverse-phase high-performance liquid chromatography (ExRP-HPLC) approach to quickly and accurately analyze the oxidation and reduction by-products of rhIL-15, which may appear during the purification processes. Firstly, we developed RP-HPLC methods which can separate rhIL-15 fractions with different levels of oxidization or reduction, respectively, and the redox status of each peak was then determined by measuring the intact mass with a high-resolution mass spectrometer (UPLC-MS). To further clarify the complex pattern of oxidization of specific residues, the peaks with various oxidation levels were digested into pieces for peptide mapping to pinpoint the exact changes of oxygen and hydrogen atoms in the rhIL-15 by-products. In addition, we performed the ExRP-HPLC and UPLC-MS analysis of partially deamidated rhIL-15 to characterize their oxidation and reduction. Our work is the first in-depth characterization of the redox by-products of rhIL-15, even for deamidated impurities. The ExRP-HPLC method we reported can facilitate the rapid and accurate quality analysis of rhIL-15, which is substantially helpful for streamlining the industrial manufacturing of rhIL-15 to better meet the demands of clinical applications. Keypoints • The oxidization and reduction rhIL-15 by-products were characterized for the first time. • The changes of oxygen and hydrogen atoms in rhIL-15 redox by-products were accurately determined by UPLC-MS. • Oxidation and reduction by-products of deamidated rhIL-15 were further analyzed.