Hormonal and immunological responses of Holstein dairy cows from late lactation to the dry period and from the dry period to early lactation

• Published in: Domestic animal endocrinology 2023-04, Vol.83, p.106790-106790, Article 106790
• Main Authors: Gao, J., Marins, T.N., Calix, J.O.S., Qi, Z., Bernard, J.K., Tao, S.
• Format: Article
• Language: English
• Subjects: Animals; Cattle; Dairy cows; Dry-off; Female; Hydrocortisone; Lactation - physiology; Leukocytes, Mononuclear; Lipopolysaccharides - pharmacology; Milk; Parturition; Prolactin; Stress hormone
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/j.domaniend.2023.106790

•In this study, based on the proliferative responses of peripheral immune cells challenged in vitro with common mitogens, we concluded that:•Cows in the final week of gestation and the first 2 wks of lactation had lower cell-mediated immune responses compared with 21 d of lactation.•Cows transitioning from late lactation to dry period maintained unchanged cell-mediated immune function. Parturition and dry-off are challenging events for dairy cows partially due to changes in endocrine responses. The aim of this experiment was to evaluate blood concentrations of cortisol and prolactin and their effects on proliferation of peripheral blood mononuclear cells (PBMC) with or without stimulation by common immune cell mitogens (lipopolysaccharide [LPS], and concanavalin A [ConA]) of multiparous dairy cows from late lactation to the dry period and from the dry period to early lactation. Two groups of cows were enrolled: cows from late lactation to the dry period enrolled at 8 d before dry-off (LTD, n = 6, days in milk at dry-off = 332 ± 41 d) and cows from the dry period to early lactation enrolled at 7 d before expected calving date (DTL, n = 7). Blood was collected on d -8, 3, 7, and 15 relatives to dry-off for LTD cows, and on d -7, 3, 7, and 21 relatives to calving for DTL cows to analyze circulating stress hormones and to isolate PBMC. The PBMC were stimulated in vitro with prolactin (PRL), hydrocortisone (HDC), LPS, ConA, PRL + LPS, PRL + ConA, HDC+LPS, and HDC + ConA to assess proliferative responses. Plasma cortisol and PRL concentrations of LTD and DTL cows were not affected by time. Regardless of time, addition of HDC reduced PBMC proliferation stimulated by LPS, but PRL had no effect. No time effect was observed for proliferation of PBMC collected from LTD cows, but PBMC collected at 21 d after calving had higher proliferative responses to LPS and ConA than those from late dry period or early lactation. In conclusion, results from this experiment confirmed the lower PBMC proliferation during the transition period from the final week of gestation to early lactation and suggested that cows transitioning from late lactation to dry period maintained unchanged cell-mediated immune function.