Quantitative analysis of culturable bacteria, levels of endotoxins, inflammatory mediators and substance P in teeth with symptomatic irreversible pulpitis and in teeth with vital normal pulp tissues

Aim To comparatively analyse the levels of culturable bacteria, endotoxins (LPS), tumour necrosis factor‐alpha (TNF‐α), interleukin‐1 beta (IL‐1β) and substance P in teeth with symptomatic irreversible pulpitis (SIP) and vital normal pulp (VNP) tissues. Methodology Thirty‐two patients were included...

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Bibliographic Details
Published in:International endodontic journal 2023-07, Vol.56 (7), p.827-836
Main Authors: Arruda‐Vasconcelos, R., Chiarelli‐Neto, V. M., Louzada, L. M., Aveiro, E., Alves‐Silva, E. G., de‐Jesus‐Soares, A., Ferraz, C. C. R., Almeida, J. F. A., Marciano, M. A., Pecorari, V. G. A., Gomes, B. P. F. A.
Format: Article
Language:English
Subjects:
Bacteria
Cross-Sectional Studies
Dental pulp
Dental Pulp - pathology
endodontics
Endotoxins
Enzyme-linked immunosorbent assay
Humans
Inflammation
Inflammation Mediators
irreversible pulpitis
Lipopolysaccharides
normal pulp
Pulpitis
Quantitative analysis
Root canals
Statistical analysis
Substance P
Tumor necrosis factor
Tumor Necrosis Factor-alpha
Tumor necrosis factor-TNF
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Summary:Aim To comparatively analyse the levels of culturable bacteria, endotoxins (LPS), tumour necrosis factor‐alpha (TNF‐α), interleukin‐1 beta (IL‐1β) and substance P in teeth with symptomatic irreversible pulpitis (SIP) and vital normal pulp (VNP) tissues. Methodology Thirty‐two patients were included (20 teeth with SIP and 12 teeth with VNP tissues) in this cross‐sectional study. Samples were collected from the full length of the root canals (microbial analysis) and periapical tissues (2 mm beyond the apex for immunological analysis), using sterile absorbent paper points. The levels of culturable bacteria (culture method), endotoxins (LAL Pyrogent 5000), TNF‐α, IL‐1β and substance P (ELISA) were assessed. The Mann–Whitney test was used for comparisons between the levels of CFU/mL, LPS, TNF‐α, IL‐1β and substance P in the SIP and VNP groups. The statistical analysis was performed with the significance level set at 5%. Results Culturable bacteria were recovered from all teeth with SIP. On the other hand, no positive cultures were observed in the VNP tissues group (p > .05). The levels of LPS were approximately four times higher in teeth with SIP than in teeth with VNP tissues (p 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.13922