A missense SNP in the proopiomelanocortin (POMC) gene is associated with growth traits in Awassi and Karakul sheep

This study was conducted to assess the association between proopiomelanocortin (POMC) gene and growth traits in Awassi and Karakul sheep. PCR-single strand conformation polymorphism (SSCP) method was utilized to assess the polymorphism of POMC PCR amplicons with body weight and length, wither and ru...

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Bibliographic Details
Published in:Animal biotechnology 2023-12, Vol.34 (9), p.4837-4850
Main Authors: Aljubouri, Thamer R. S., Al-Shuhaib, Mohammed Baqur S.
Format: Article
Language:English
Subjects:
Animals
Awassi
Body length
Body weight
Body Weight - genetics
Chest
Circumferences
Conformation
damaging effect
Gene polymorphism
Genotype
Genotypes
Glycine
Karakul
Lamb
Phenotype
Polymorphism
Polymorphism, Single Nucleotide - genetics
Polymorphism, Single-Stranded Conformational
POMC
Pro-Opiomelanocortin - genetics
Proopiomelanocortin
Sheep
Sheep - genetics
Sheep, Domestic - genetics
Single-nucleotide polymorphism
SNP
Structural stability
Structure-function relationships
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Summary:This study was conducted to assess the association between proopiomelanocortin (POMC) gene and growth traits in Awassi and Karakul sheep. PCR-single strand conformation polymorphism (SSCP) method was utilized to assess the polymorphism of POMC PCR amplicons with body weight and length, wither and rump height, chest and abdominal circumference measured at birth, 3, 6, 9, and 12 months intervals. Only one missense SNP (rs424417456:C > A) was detected in exon-2, in which glycine was converted to cysteine in the 65th position in POMC (p.65Gly > Cys). rs424417456 SNP showed significant associations with all growth traits in the third, sixth, ninth, and twelfth months. At the age of 3 months onward, lambs with CC genotype showed higher body weight, body length, wither and rump heights, and chest and abdominal circumferences than lambs with CA and AA genotypes, respectively. Prediction analyses indicated a deleterious effect of p.65Gly > Cys on POMC structure, function, and stability. Owing to the strong correlation between rs424417456:CC and better growth characteristics, this genotype is proposed as a promising marker to enhance growth traits in Awassi and Karakul sheep. The predicted damaging effects caused by rs424417456:CA and rs424417456:AA genotypes may entail a putative mechanism through which lambs with these genotypes exhibit lower growth traits.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2023.2197469