MicroRNA-483-3p Inhibitor Ameliorates Sepsis-Induced Intestinal Injury by Attenuating Cell Apoptosis and Cytotoxicity Via Regulating HIPK2

Sepsis is a life-threatening syndrome that can result in multi-organ dysfunction. MicroRNA (miR)-483-3p was previously demonstrated to be upregulated in sepsis patients; however, its specific functions in sepsis-triggered intestinal injury remain unclarified. Human intestinal epithelial NCM460 cell...

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Bibliographic Details
Published in:Molecular biotechnology 2024-02, Vol.66 (2), p.233-240
Main Authors: Wang, Zhen, Qin, Xuemei, Yuan, Jin, Yin, Hongzhen, Qu, Rui, Zhong, Changshun, Ding, Wei
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biological Assay
Biological Techniques
Biotechnology
Carrier Proteins
Cell Biology
Chemistry
Chemistry and Materials Science
Cytotoxicity
Diamines
DNA nucleotidylexotransferase
Fatty acid-binding protein
HIPK2 protein
Homeobox
Human Genetics
Humans
Injuries
Intestine
Kinases
L-Lactate dehydrogenase
Lactate dehydrogenase
Lipopolysaccharides
Lipopolysaccharides - pharmacology
MicroRNAs
MicroRNAs - genetics
miRNA
Original Paper
Polymerase chain reaction
Protein Science
Protein Serine-Threonine Kinases
Proteins
Sepsis
Sepsis - complications
Sepsis - genetics
Toxicity
Western blotting
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Summary:Sepsis is a life-threatening syndrome that can result in multi-organ dysfunction. MicroRNA (miR)-483-3p was previously demonstrated to be upregulated in sepsis patients; however, its specific functions in sepsis-triggered intestinal injury remain unclarified. Human intestinal epithelial NCM460 cell line was stimulated with lipopolysaccharide (LPS) to mimic sepsis-induced intestinal injury in vitro. Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining was utilized for examining cell apoptosis. Western blotting and real time quantitative polymerase chain reaction (RT-qPCR) were used for detecting molecular protein and RNA levels. LPS-induced cytotoxicity was determined by measuring concentrations of lactate dehydrogenase (LDH), diamine oxidase (DAO) and fatty acid binding protein 2 (FABP2). Luciferase reporter assay was utilized for verifying the interaction between miR-483-3p and homeodomain interacting protein kinase 2 (HIPK2). Inhibiting miR-483-3p alleviates LPS-triggered NCM460 cell apoptosis and cytotoxicity. miR-483-3p targeted HIPK2 in LPS-stimulated NCM460 cells. Knockdown of HIPK2 reversed the above effects mediated by miR-483-3p inhibitor. Inhibiting miR-483-3p ameliorates LPS-triggered apoptosis and cytotoxicity by targeting HIPK2.
ISSN:1073-6085
1559-0305
DOI:10.1007/s12033-023-00734-x