Single von Willebrand factor C-domain protein confers host defense against white spot syndrome virus by functioning as a pattern recognition receptor in Macrobrachium nipponense

• Published in: International journal of biological macromolecules 2023-06, Vol.241, p.124520, Article 124520
• Main Authors: Qin, Nan, Li, Muyi, Zhang, Han, Li, Feifei, Guo, Xinrui, Wu, Mengjia, Zhang, Qingli, Tang, Ting, Liu, Fengsong
• Format: Article
• Language: English
• Subjects: Animals; Antiviral immunity; Arthropod Proteins - genetics; Arthropod Proteins - metabolism; blood coagulation factors; Calmodulin; genes; hemocytes; humoral immunity; interferons; Interferons - metabolism; Janus Kinases - metabolism; Macrobrachium nipponense; mortality; nerve tissue; Nodaviridae; nutritional status; Palaemonidae - genetics; Pattern recognition receptor; Penaeidae - genetics; Penaeidae - metabolism; Phagocytosis; precipitin tests; Receptors, Pattern Recognition - genetics; Receptors, Pattern Recognition - metabolism; shrimp; Signal Transduction; Single von Willebrand factor C-domain protein; STAT Transcription Factors - metabolism; transcription factors; von Willebrand Factor - metabolism; White spot syndrome virus; White spot syndrome virus 1 - genetics
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.124520

The single von Willebrand factor C-domain proteins (SVWCs), also known as Vago, are primarily found in arthropods. Their expression was induced by nutritional status, bacterial and viral infections. Despite the prominence of SVWCs in antiviral immunity, the detailed molecular mechanisms remain poorly explained. SVWC has been proposed to elicit antiviral activities through its function as an interferon analog. In contrast, herein, we illustrate that an SVWC homolog from Macrobrachium nipponense (MnSVWC) confers host defense against white spot syndrome virus (WSSV) and covert mortality nodavirus (CMNV) as a pattern recognition receptor (PRR). qRT-PCR analyses demonstrated that the expression of MnSVWC was enhanced upon WSSV infection in all detected tissues, including gills, nerve cords, and hemocytes. Coating WSSV with recombinant MnSVWC (rMnSVWC) promoted the phagocytic activity of hemocytes and subsequent clearance of invasive WSSV from the prawn. On the other hand, the knockdown of MnSVWC with RNAi improved the proliferation ability of WSSV and CMNV in the prawn. Analysis of ELISA and Co-immunoprecipitation (Co-IP) showed that rMnSVWC could bind WSSV by interacting with the vesicle proteins VP26 and VP28. Co-IP analysis verified the interaction between MnSVWC and calmodulin, which implies a vesicle protein-SVWC-calmodulin-clathrin-dependent mechanism underlying the hemocyte-mediated phagocytosis against WSSV. Subsequently, MnSVWC was recognized to activate the expression of transcription factor STAT and an interferon-stimulating gene Viperin, illustrating its involvement in modulating humoral immunity via activation of the JAK/STAT pathway after WSSV infection. These findings indicate that MnSVWC could bind to WSSV as a PRR and participate in the promotion of hemocyte-mediated phagocytosis and the activation of the JAK/STAT pathway in prawns.