Phase partitioning of mercury, arsenic, selenium, and cadmium in Chlamydomonas reinhardtii and Arthrospira maxima microcosms

• Published in: Environmental pollution (1987) 2023-07, Vol.329, p.121679-121679, Article 121679
• Main Authors: Lown, Livia, Vernaz, Joshua E., Dunham-Cheatham, Sarrah M., Gustin, Mae S., Hiibel, Sage R.
• Format: Article
• Language: English
• Subjects: Aquaculture; Arsenic - metabolism; Arthrospira maxima; Biogeochemical cycling; Biomass; Cadmium - metabolism; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - metabolism; Gases; Humans; Mercury - metabolism; Microalgae - metabolism; Selenium - metabolism; Spirulina; Wastewater
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2023.121679

As the global human population increases, demand for protein will surpass our current production ability without an increase in land use or intensification. Microalgae cultivation offers a high yield of protein, and utilization of wastewater from municipal or agricultural sources in place of freshwater for microalgae aquaculture may increase the sustainability of this practice. However, wastewater from municipal and agricultural sources may contain contaminants, such as mercury (Hg), cadmium (Cd), selenium (Se), and arsenic (As). Association of these elements with algal biomass may present an exposure risk to product consumers, while volatilization may present an exposure hazard to industry workers. Thus, the partitioning of these elements should be evaluated before wastewater can be confidently used in an aquaculture setting. This study explored the potential for exposure associated with Arthrospira maxima and Chlamydomonas reinhardtii aquaculture in medium contaminated with 0.33 μg Hg L−1, 60 μg As L−1, 554 μg Se L−1, and 30 μg Cd L−1. Gaseous effluent from microalgae aquaculture was analyzed for Hg, As, Se, and Cd to quantify volatilization. A mass balance approach was used to describe the partitioning of elements between the biomass, medium, and gas phases at the end of exponential growth. Contaminants were recovered predominantly in medium and biomass, regardless of microalgae strain. In the case of Hg, 48 ± 2% was associated with A. maxima biomass and 55 ± 8% with C. reinhardtii when Hg was present as the only contaminant, but this increased to 85 ± 11% in C. reinhardtii biomass when As, Se, and Cd were also present. A small and highly variable abiotic volatilization of Hg was observed in the gas phase of both A. maxima and C. reinhardtii cultures. Evidence presented herein suggests that utilizing wastewater containing Hg, Cd, Se, and As for microalgae cultivation may present health hazards to consumers. [Display omitted] •Microalgae aquaculture in wastewater may provide a sustainable source of protein.•Metal(oid) volatilization or uptake by microalgae may expose consumers or workers.•Chlamydomonas reinhardtii and Arthrospira maxima did not volatize Hg, As, Se, or Cd.•Significant amounts of Hg were recovered in biomass of both microalgae.