Species composition of root-associated mycobiome of ruderal invasive Anthemis cotula L. varies with elevation in Kashmir Himalaya

• Published in: International microbiology 2023-11, Vol.26 (4), p.1053-1071
• Main Authors: Afshana, Reshi, Zafar A., Shah, Manzoor A., Malik, Rayees A., Rashid, Irfan
• Format: Article
• Language: English
• Subjects: Anthemis cotula; Applied Microbiology; Arbuscular mycorrhizas; Biomass; Biomedical and Life Sciences; Carbon dioxide; Carbon dioxide concentration; Composition; Eukaryotic Microbiology; Fungi; Genera; Host plants; Introduced species; Invasive plants; Invasive species; Life Sciences; Medical Microbiology; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microorganisms; Nonnative species; Plant species; Plants (botany); Roots; Soil microorganisms; Species composition; Species diversity
• ISSN: 1618-1905; 1139-6709; 1618-1905
• DOI: 10.1007/s10123-023-00359-9

  Investigating the microbial communities associated with invasive plant species can provide insights into how these species establish and thrive in new environments. Here, we explored the fungal species associated with the roots of the invasive species Anthemis cotula L. at 12 sites with varying elevations in the Kashmir Himalaya. Illumina MiSeq platform was used to identify the species composition, diversity, and guild structure of these root-associated fungi. The study found a total of 706 fungal operational taxonomic units (OTUs) belonging to 8 phyla, 20 classes, 53 orders, 109 families, and 160 genera associated with roots of A. cotula , with the most common genus being Funneliformis . Arbuscular mycorrhizal fungi (AMF) constituted the largest guild at higher elevations. The study also revealed that out of the 12 OTUs comprising the core mycobiome, 4 OTUs constituted the stable component while the remaining 8 OTUs comprised the dynamic component. While α-diversity did not vary across sites, significant variation was noted in β-diversity. The study confirmed the facilitative role of the microbiome through a greenhouse trial in which a significant effect of soil microbiome on height, shoot biomass, root biomass, number of flower heads, and internal CO 2 concentration of the host plant was observed. The study indicates that diverse fungal mutualists get associated with this invasive alien species even in nutrient-rich ruderal habitats and may be contributing to its spread into higher elevations. This study highlights the importance of understanding the role of root-associated fungi in invasion dynamics and the potential use of mycobiome management strategies to control invasive species.