Osteogenic differentiation of human adipose-derived mesenchymal stem cells in a bisphosphonate-functionalized polycaprolactone/gelatin scaffold

Recent trends in bone tissue engineering have focused on the development of biomimetic constructs with appropriate mechanical and physiochemical properties. Here, we report the fabrication of an innovative biomaterial scaffold based on a new bisphosphonate-containing synthetic polymer combined with...

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Bibliographic Details
Published in:International journal of biological macromolecules 2023-06, Vol.241, p.124573-124573, Article 124573
Main Authors: Safari, Banafsheh, Aghazadeh, Marziyeh, Aghanejad, Ayuob
Format: Article
Language:English
Subjects:
Adipose-derived mesenchymal stem cell
Cell Differentiation
Diphosphonates
Functionalized polycaprolactone
Gelatin - chemistry
Humans
Mesenchymal Stem Cells
Osteogenesis
Polyesters - chemistry
Polymers
Scaffold
Theranostic
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Zoledronate
Zoledronic Acid
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Summary:Recent trends in bone tissue engineering have focused on the development of biomimetic constructs with appropriate mechanical and physiochemical properties. Here, we report the fabrication of an innovative biomaterial scaffold based on a new bisphosphonate-containing synthetic polymer combined with gelatin. To this end, zoledronate (ZA)-functionalized polycaprolactone (PCL-ZA) was synthesized by a chemical grafting reaction. After adding gelatin to the PCL-ZA polymer solution, the porous PCL-ZA/gelatin scaffold was fabricated by the freeze-casting method. A scaffold with aligned pores and a porosity of 82.04 % was obtained. During in vitro biodegradability test, 49 % of its initial weight lost after 5 weeks. The elastic modulus of the PCL-ZA/gelatin scaffold was 31.4 MPa, and its tensile strength was 4.2 MPa. Based on the results of MTT assay, the scaffold had good cytocompatibility with human Adipose-Derived Mesenchymal Stem Cells (hADMSCs). Furthermore, cells grown in PCL-ZA/gelatin scaffold showed the highest mineralization and ALP activity compared to other test groups. Results of the RT-PCR test revealed that RUNX2, COL 1A1, and OCN genes were expressed in PCL-ZA/gelatin scaffold at the highest level, suggesting its good osteoinductive capacity. These results revealed that PCL-ZA/gelatin scaffold could be considered a proper biomimetic platform for bone tissue engineering.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.124573