The Phylogenetic Study of the CRISPR-Cas System in Enterobacteriaceae

The C lustered R egularly I nterspaced S hort P alindromic R epeats (CRISPR)- C RISPR- as sociated (Cas) system is a bacterial and archaeal adaptive immune system undergoing rapid multifaceted evolution. This evolution plausibly occurs due to the genetic exchanges of complete loci or individual enti...

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Published in:Current microbiology 2023-06, Vol.80 (6), p.196-196, Article 196
Main Authors: Kushwaha, Simran Krishnakant, Kumar, Aryahi A., Gupta, Hardik, Marathe, Sandhya Amol
Format: Article
Language:English
Subjects:
adaptive immunity
Adaptive systems
Archaea
Biological evolution
Biomedical and Life Sciences
Biotechnology
CRISPR
CRISPR-Cas Systems
Enterobacteriaceae
Enterobacteriaceae - genetics
Evolution
genes
Immune system
Life Sciences
loci
Microbiology
Phylogeny
Spacers
species
Strains (organisms)
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Summary:The C lustered R egularly I nterspaced S hort P alindromic R epeats (CRISPR)- C RISPR- as sociated (Cas) system is a bacterial and archaeal adaptive immune system undergoing rapid multifaceted evolution. This evolution plausibly occurs due to the genetic exchanges of complete loci or individual entities. Here, we systematically investigate the evolutionary framework of the CRISPR-Cas system in six Enterobacteriaceae species and its evolutionary association with housekeeping genes as determined by the gyrB phenogram. The strains show high variability in the cas3 gene and the CRISPR1 locus among the closely related Enterobacteriaceae species, hinting at a series of genetic exchanges. The CRISPR leader is conserved, especially toward the distal end, and could be a core region of the leader. The spacers are conserved within the strains of most species, while some strains show unique sets of spacers . However, inter-species spacer conservation was rarely observed. For a considerable proportion of these spacers, protospacer sources were not detected. These results advance our understanding of the dynamics of the CRISPR-Cas system; however, the biological functions are yet to be characterised. Graphical Abstract
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-023-03298-w