Shisa reduces the sensitivity of homomeric RDL channel to GABA in the two-spotted spider mite, Tetranychus urticae Koch

• Published in: Pesticide biochemistry and physiology 2023-05, Vol.192, p.105414-105414, Article 105414
• Main Authors: Zhan, Enling, Jiang, Jie, Wang, Ying, Zhang, Kexin, Tang, Tao, Chen, Yiqu, Jia, Zhongqiang, Wang, Qiuxia, Zhao, Chunqing
• Format: Article
• Language: English
• Subjects: Amino Acids - metabolism; Animals; Auxiliary subunit; Chloride channel; GABAergic; Insecticides - metabolism; Mammals - metabolism; pGH19; Phylogeny; Receptors, GABA - chemistry; Tetranychidae - genetics; Tetranychidae - metabolism; Z-DOCK
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2023.105414

The γ-aminobutyric acid receptors (GABARs) mediate fast inhibitory transmission in central nervous system of insects and are important targets of insecticides. An auxiliary subunit, Shisa7, was identified in mammals as a single-passing transmembrane protein. However, the homology gene(s) of Shisa in invertebrates has not been reported to date. In the present study, a homolog Shisa gene was identified from the two-spotted spider mite, Tetranychus urticae Koch. Its open reading frame had 927 base pairs and encoded 308 amino acid residues, which has a typical Shisa domain at 13th-181st amino acid residues. According to the phylogenetic tree, the invertebrate Shisa was categorized apart with those of vertebrate, and TuShisa showed closest relationship with the Shisa9 of velvet mite, Dinothrombium tinctorium (L.). In the electrophysiological assay with two-electrode voltage clamp, the GABA-activated TuRDL channel was functionally formed in the Africa clawed frog Xenopus laevis (Daudin) oocytes (EC50 = 53.34 μM). No GABA-activated current could be observed in TuShisa-expressed oocytes, whereas TuShisa could reduce the sensitivity of TuRDL/TuShisa (mass ratio of 1: 4) channel to GABA. The homology structural models of TuRDL and TuShisa were built by the SWISS-MODEL server, their interaction was predicted using Z-DOCK and three predicted hydrogen bonds and interface residues were analysed by PyMOL. Meanwhile, the key interface residues of TuShisa affected the stability of complex were calculated by Discovery Studio 2019. In conclusion, the TuShisa, as the first reported invertebrate Shisa, was explored and functionally examined as the GABARs auxiliary subunit. Our findings provide a basis for research of invertebrate Shisa. [Display omitted] •TuShisa was first isolated from two-spotted spider mite Tetranychus urticae Koch.•TuShisa could reduce the sensitivity of TuRDL channel expressed in Xenopus laevis oocytes to GABA.•TuShisa interacted with TuRDL by three hydrogen bonds.•GLY29 and GLY18 of TuShisa were two key interface residues affected the stability of TuRDL-TuShisa complex.