Anticandidal Efficacy of Erythrosine with Nano-TiO2 and Blue LED-Mediated Photodynamic Therapy against Candida albicans Biofilms on Acrylic Resin: A Preliminary Study

• Published in: European journal of dentistry 2024-02, Vol.18 (1), p.273-280
• Main Authors: Damrongrungruang, Teerasak, Puasiri, Subin, Vongtavatchai, Vichakorn, Saeng-On, Chatchai, Petcharapiruch, Teeruch, Teerakapong, Aroon, Sangpanya, Angkhana
• Format: Article
• Language: English
• ISSN: 1305-7456; 1305-7464
• DOI: 10.1055/s-0043-1768165

Incorporating an enhancer such as nano-titanium dioxide into antimicrobial photodynamic therapy can improve treatment outcome.This study aimed to compare the anticandidal efficacy of photodynamic therapy by erythrosine with nano-titanium dioxide (nano-TiO ) stimulated by a blue light emitting diode with three standard dental antifungal agents.   biofilms on acrylic resin plates were treated for 15 minutes with either nystatin, fluconazole, Polident, 220µM erythrosine + 1% (w/w) nano-TiO  + 15 J/cm blue light photodynamic therapy (Ery PDT), or distilled water. For the Ery PDT group, blue light was applied for 1 minute after incubation. After 1, 3, and 6 hours, the colony forming units in log10 (log CFU/mL) were compared. The ultrastructure of on the acrylic resin plates treated with erythrosine + nano-TiO  + blue light was examined using transmission electron microscopy at magnification of 30,000x.  After 1 hour, nystatin, Polident, and Ery PDT indifferently inhibited . At 6 hours, Ery PDT reduced the number of viable in biofilms by 0.28log CFU/mL, which was equal to the effect of fluconazole and Polident. Transmission electron microscopy demonstrated that Ery PDT altered the cell morphology by inducing cell wall/membrane rupture.  Photodynamic therapy with erythrosine + nano-TiO  + blue light at low light power density (15 J/cm ) was as effective at inhibiting biofilm on acrylic resin as fluconazole and Polident.