Investigation of low and high dose rate X-ray effects on histopathological changes and prognostic importance of Ki-67 in laryngeal cancer radiotherapy

• Published in: Applied radiation and isotopes 2023-07, Vol.197, p.110823-110823, Article 110823
• Main Authors: Aras, Serhat, Ozkanli, Seyma, Erdem, Esra, Gokalp, Sevtap, Erdogan, Ceren Ezgi
• Format: Article
• Language: English
• Subjects: Animals; Flattening filter free; Histopathology; Ki-67; Ki-67 Antigen; Laryngeal cancer; Laryngeal Neoplasms - radiotherapy; Mice; Mice, SCID; Prognosis; Radiotherapy; Radiotherapy Dosage; Radiotherapy Planning, Computer-Assisted - methods; Radiotherapy, Intensity-Modulated - methods; X-Rays
• ISSN: 0969-8043; 1872-9800
• DOI: 10.1016/j.apradiso.2023.110823

The aim of this study is to analyze the effect on histopathological changes and Ki-67 expression levels of Flattening Filter (FF) and Flattening Filter Free (FFF) beams to investigate the radiobiological mechanisms underlying laryngeal cancer (LCa) post-radiotherapy (RT) on mice models. Forty adult NOD SCID gamma (NSG) mice models were randomly divided into four groups; the sham, LCa, FF-RT and FFF-RT groups. The head and neck region of mice in FF-RT and FFF-RT groups (LCa plus RT groups) were irradiated with a single dose of 18 Gy at 400 MU/min and 1400 MU/min. The NSG mice received radiotherapy 30 days after tumor transplantation and sacrificed 2 days after radiotherapy for analysis of histopathology parameters and K-67 expression levels. Comparing the LCa, FF-RT and FFF-RT groups with the sham group, statistically significant differences were observed in histopathological parameters depending on tumor tissue and dose rate (p