Carbon quantum dots as immune modulatory therapy in a Sjögren's syndrome mouse model

• Published in: Oral diseases 2024-04, Vol.30 (3), p.1183-1197
• Main Authors: Fu, Cuicui, Qin, Xiaoyun, Shao, Wenlong, Zhang, Jin, Zhang, Ting, Yang, Jiaqi, Ding, Chong, Song, Yeqing, Ge, Xuejun, Wu, Gang, Bikker, Floris J., Jiang, Nan
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Carbon; Carbon - pharmacology; Carbon - therapeutic use; carbon quantum dots; CD3 antigen; CD4 antigen; Cell viability; Cytotoxicity; Diabetes mellitus; Disease Models, Animal; Female; Foxp3 protein; Glands; Immunomodulation; immunomodulatory role; Inflammation; Lymphocytes T; Mice; Mice, Inbred NOD; Optical properties; Quantum dots; Quantum Dots - therapeutic use; Saliva; Saliva - chemistry; Saliva - metabolism; Sjogren's syndrome; Sjogren's Syndrome - drug therapy; Sjogren's Syndrome - immunology; Sjögren's syndrome; Submandibular Gland - drug effects; Submandibular Gland - pathology; T cells
• ISSN: 1354-523X; 1601-0825; 1601-0825
• DOI: 10.1111/odi.14603

Objectives The objective of the study was to evaluate the therapeutic effects of carbon quantum dots (CQDs) in immunomodulation on non‐obese diabetic (NOD) mice, as the model for Sjögren's syndrome (SS). Methods Carbon quantum dots were generated from Setaria viridis via a hydrothermal process. Their toxic effects were tested by cell viability and blood chemistry analysis, meanwhile therapeutic effects were investigated in NOD mice in the aspects of saliva flow, histology, and immune cell distribution. Results Carbon quantum dots, with rich surface chemistry and unique optical properties, showed non‐cytotoxicity in vitro or no damage in vivo. Intravenously applied CQDs alleviated inflammation in the submandibular glands in NOD mice after 6‐week treatments. The inflammatory area index and focus score were significantly decreased in CQD‐treated mice. Besides, the levels of anti‐SSA and anti‐SSB were decreased in the presence of CQDs. The stimulated saliva flow rates and weight of submandibular glands were significantly increased in CQD‐treated mice by reducing the apoptosis of cells. The CD3+ and CD4+ T cells distributed around the ducts of submandibular glands were significantly decreased, while the percentage of Foxp3+ cells was higher in CQD‐treated mice than that in the control group. Conclusions Our findings suggest that CQDs may ameliorate the dysregulated immune processes in NOD mice.