Promethazine inhibits efflux, enhances antifungal susceptibility and disrupts biofilm structure and functioning in Trichosporon

Trichosporon spp. are emerging opportunistic fungi associated with invasive infections, especially in patients with haematological malignancies. The present study investigated the in vitro inhibition of efflux pumps by promethazine (PMZ) as a strategy to control T. asahii and T. inkin. Planktonic ce...

Full description

Saved in:
Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2023-02, Vol.39 (2), p.218-230
Main Authors: Aguiar, Ana Luiza Ribeiro, Silva, Bruno Nascimento da, Fiallos, Nicole de Mello, Pereira, Lívia Maria Galdino, Silva, Maria Laína, Souza, Pedro Freitas Santos Manzi de, Portela, Fernando Victor Monteiro, Sidrim, José Júlio Costa, Rocha, Marcos Fábio Gadelha, Castelo-Branco, Débora Souza Collares Maia, Cordeiro, Rossana de Aguiar
Format: Article
Language:English
Subjects:
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Antifungal resistance
Biofilms
Carbohydrates
Efflux
efflux pumps
Fungi
Fungicides
Humans
Malignancy
Microbial Sensitivity Tests
persister cells
Plankton
Planktonic cells
Promethazine
Promethazine - metabolism
Promethazine - pharmacology
Pumps
Trichosporon
Trichosporonosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Trichosporon spp. are emerging opportunistic fungi associated with invasive infections, especially in patients with haematological malignancies. The present study investigated the in vitro inhibition of efflux pumps by promethazine (PMZ) as a strategy to control T. asahii and T. inkin. Planktonic cells were evaluated for antifungal susceptibility to PMZ, as well as inhibition of efflux. The effect of PMZ was also studied in Trichosporon biofilms. PMZ inhibited T. asahii and T. inkin planktonic cells at concentrations ranging from 32 to 256 μg   ml -1 . Subinhibitory concentrations of PMZ inhibited efflux activity in Trichosporon. Biofilms were completely eradicated by PMZ. PMZ potentiated the action of antifungals, affected the morphology, changed the amount of carbohydrates and proteins and reduced the amount of persister cells inside biofilms. The results showed indirect evidences of the occurrence of efflux pumps in Trichosporon and opens a perspective for the use of this target in the control of trichosporonosis.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2023.2202315