Notch pathway is required for protection against heat stress in spermatogonial stem cells in medaka

• Published in: Fish physiology and biochemistry 2023-06, Vol.49 (3), p.487-500
• Main Authors: Moreno Acosta, Omar D., Boan, Agustín F., Hattori, Ricardo S., Fernandino, Juan Ignacio
• Format: Article
• Language: English
• Subjects: adults; ambient temperature; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Apoptosis; Biomedical and Life Sciences; Cell Differentiation; Fertility; fish; Freshwater & Marine Ecology; Freshwater fishes; Gametes; Gametocytes; Genes; Heat; Heat stress; Heat tolerance; Heat treatment; Heat treatments; Heat-Shock Response; Histology; Life Sciences; Ligands; Male; Metaphase; Morphology; Oryzias; Presenilin 2; Protection; protein subunits; Receptors; Secretase; Somatic cells; Spermatids; Spermatocytes; Spermatogenesis; Spermatogenesis - genetics; Spermatogonia; Spermatogonia - physiology; Stem Cells; Survival; Temperature effects; testes; Testis - metabolism; Thermal stress; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-023-01200-w

Gamete production is a fundamental process for reproduction; however, exposure to stress, such as increased environmental temperature, can decrease or even interrupt this process, affecting fertility. Thus, the survival of spermatogonial stem cells (SSCs) is crucial for the recovery of spermatogenesis upon stressful situations. Here, we show that the Notch pathway is implicated in such survival, by protecting the SSCs against thermal stress. First, we corroborated the impairment of spermatogenesis under heat stress in medaka, observing an arrest in metaphase I at 10 days of heat treatment, an increase in the number of spermatocytes, and downregulation of ndrg1b and sycp3 . In addition, at 30 days of treatment, an interruption of spermatogenesis was observed with a strong loss of spermatocytes and spermatids. Then, the exposure of adult males to thermal stress condition induced apoptosis mainly in spermatogenic and supporting somatic cells, with the exception of the germinal region, where SSCs are located. Concomitantly, the Notch pathway–related genes were upregulated, including the ligands (dll4, jag1-2) and receptors (notch1a-3). Moreover, during thermal stress presenilin enhancer-2 (pen-2), the catalytic subunit of γ-secretase complex of the Notch pathway was restricted to the germinal region of the medaka testis, observed in somatic cells surrounding type A spermatogonia (SGa). The importance of Notch pathway was further supported by an ex vivo approach, in which the inhibition of this pathway activity induced a loss of SSCs. Overall, this study supports the importance of Notch pathways for the protection of SSCs under chronic thermal stress.