Transcriptomic analysis reveals gene expression changes in peripheral white blood cells of cows after embryo transfer: Implications for pregnancy tolerance

Most embryo losses occur in the first trimester of pregnancy in cows and include losses following embryo transfer. There is a resulting negative economic impact on cattle production systems when this occurs. Cellular and molecular mechanisms behind the maternal immune response to the growing embryo...

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Bibliographic Details
Published in:Reproduction in domestic animals 2023-07, Vol.58 (7), p.946-954
Main Authors: De los Santos, Jorge A., Andrade, João Paulo N., Cangiano, L. R., Iriarte, Andres, Peñagaricano, Francisco, Parrish, John J.
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Blood coagulation
bovine interferon‐stimulated genes
Cattle
Cattle - genetics
Cattle production
Cell adhesion
Chemokines
Chemotaxis
early pregnancy diagnosis
Economic impact
Ectoparasites
Embryo transfer
Embryo Transfer - methods
Embryo Transfer - veterinary
Embryos
Female
Gene expression
Genes
immune regulation
Immune response
Immune system
Immune Tolerance - genetics
Immunological tolerance
Impact analysis
Inflammation
Inflammatory response
Leukocytes
Molecular modelling
Pregnancy
Transcriptome
Transcriptomes
Transcriptomics
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Summary:Most embryo losses occur in the first trimester of pregnancy in cows and include losses following embryo transfer. There is a resulting negative economic impact on cattle production systems when this occurs. Cellular and molecular mechanisms behind the maternal immune response to the growing embryo have not been fully characterized. The objective of this study was to examine the gene expression profiles of peripheral white blood cells (PWBCs) from pregnant cows 21 days after an embryo was transferred, and cows that were treated equally but lost the embryo. Specifically, we obtained and compared the transcriptome of PWBC from heifers that became pregnant at day 21 (N = 5) or failed to become pregnant after the embryo transfer (N = 5). Sequencing data can be accessed by Gene Expression Omnibus (GEO) with the accession number GSE210665. A total of 13,167 genes were evaluated for differential expression between groups. A total of 682 genes showed differential expression (p‐value
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.14371