Engineering of clarithromycin loaded stimulus responsive dissolving microneedle patches for the treatment of biofilms

[Display omitted] This study aimed to fabricate clarithromycin laden Eudragit S-100-based microfibers (MF), microfibers coated film (MB), clarithromycin loaded polyvinyl pyrollidone, hyaluronic acid and sorbitol-based dissolving microneedle patches (CP) and microfibers coated microneedle patches (MP...

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Published in:International journal of pharmaceutics 2023-06, Vol.640, p.123003-123003, Article 123003
Main Authors: Zafar, Saman, Arshad, Muhammad Sohail, Rana, Sadia Jafar, Patel, Mohammed, Yousef, Bushra, Ahmad, Zeeshan
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Antibiofilm
Biofilms
Clarithromycin - pharmacology
Drug Delivery Systems
Humans
Hyaluronic Acid - chemistry
Infected cutaneous wounds
Microfibers
Microneedles
Needles
Staphylococcal Infections
Staphylococcus aureus
Stimulus responsive
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Summary:[Display omitted] This study aimed to fabricate clarithromycin laden Eudragit S-100-based microfibers (MF), microfibers coated film (MB), clarithromycin loaded polyvinyl pyrollidone, hyaluronic acid and sorbitol-based dissolving microneedle patches (CP) and microfibers coated microneedle patches (MP). Morphological and phase analysis of formulations were carried out by scanning electron microscopy and differential scanning calorimetry, X-ray diffraction, respectively. Substrate liquefaction test, in vitro drug release, antimicrobial assay and in vivo antibiofilm studies were performed. MF exhibited a uniform surface and interconnected network. Morphological analysis of CP revealed sharp-tipped and uniform-surfaced microstructures. Clarithromycin was incorporated within MF and CP as amorphous solid. Liquefaction test indicated hyaluronate lyase enzyme responsiveness of hyaluronic acid. Fibers-based formulations (MF, MB and MP) provided an alkaline pH (7.4) responsive drug release; ∼79 %, ∼78 % and ∼81 %, respectively within 2 h. CP showed a drug release of ∼82 % within 2 h. MP showed ∼13 % larger inhibitory zone against Staphylococcus aureus (S. aureus) as compared to MB and CP. A relatively rapid eradication of S. aureus in infected wounds and subsequent skin regeneration was observed following MP application as compared to MB and CP indicating its usefulness for the management of microbial biofilms.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2023.123003