Construction of PEGylated chlorin e6@CuS-Pt theranostic nanoplatforms for nanozymes-enhanced photodynamic-photothermal therapy

• Published in: Journal of colloid and interface science 2023-09, Vol.645, p.122-132
• Main Authors: Sheng, Yangyi, Ren, Qian, Tao, Cheng, Wen, Mei, Qu, Pu, Yu, Nuo, Li, Maoquan, Chen, Zhigang, Xie, Xiaoyun
• Format: Article
• Language: English
• Subjects: Animals; Ce6@CuS-Pt/PEG nanoplatforms; Cell Line, Tumor; Hydrogen Peroxide; Mice; Nanoparticles - therapeutic use; Nanospheres; Nanozymes; Photochemotherapy; Photodynamic therapy; Photosensitizing Agents; Photothermal Therapy; Polyethylene Glycols; Porphyrins - pharmacology; Precision Medicine; Tumor
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2023.04.092

The Ce6@CuS-Pt/PEG nanoparticles have been prepared by firstly synthesizing CuS, then decorating Pt nanozymes and PEG, and finally loading Ce6. The Ce6@CuS-Pt/PEG nanoplatform exhibited efficient nanozyme-enhanced PDT-PTT combined therapy. [Display omitted] •Ce6@CuS-Pt/PEG NPs with high thermal conversion efficiency for photothermal therapy.•Pt nanozymes assisted photodynamic therapy.•Obvious inhibition of tumor growth due to nanozyme-enhanced PDT-PTT. Multifunctional nanoagents with photodynamic therapy (PDT) and photothermal therapy (PTT) functions have shown great promise for cancer treatment, while the design and synthesis of efficient nanoagents remain a challenge. To realize nanozyme-enhanced PDT-PTT combined therapy, herein we have synthesized the Ce6@CuS-Pt/PEG nanoplatforms as a model of efficient nanoagents. Hollow CuS nanospheres with an average diameter of ∼ 200 nm are first synthesized through vulcanization using Cu2O as the precursor. Subsequently, CuS nanospheres are surface-decorated with Pt nanoparticles (NPs) as nanozyme via an in-situ reduction route, followed by modifying the DSPE-PEG5000 and loading the photosensitizer Chlorin e6 (Ce6). The obtained Ce6@CuS-Pt/PEG NPs exhibit high photothermal conversion efficiency (43.08%), good singlet oxygen (1O2) generation ability, and good physiological stability. In addition, Ce6@CuS-Pt/PEG NPs show good catalytic performance due to the presence of Pt nanozyme, which can effectively convert H2O2 to O2 and significantly enhance the production of cytotoxic 1O2. When Ce6@CuS-Pt/PEG NPs dispersion is injected into mice, the tumors can be wholly suppressed owing to nanozyme-enhanced PDT-PTT combined therapy, providing better therapeutic effects compared to single-mode phototherapy. Thus, the present Ce6@CuS-Pt/PEG NPs can act as an efficient multifunctional nanoplatform for tumor therapy.