Ciona robusta macrophage migration inhibitory factor (Mif1 and Mif2) genes are differentially regulated in the lipopolysaccharide-challenged pharynx

The effects of lipopolysaccharide (LPS) on Mif (macrophage migration inhibitory factor) gene expression in the pharynx (haemapoetic tissue) of Ciona robusta were investigated using quantitative reverse-transcription PCR (qRT-PCR) and in situ hybridisation (ISH). To verify the induction of an inflamm...

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Bibliographic Details
Published in:Journal of fish biology 2023-09, Vol.103 (3), p.727-730
Main Authors: Dumas, Francesca, Mauro, Manuela, Vazzana, Mirella, Arizza, Vincenzo, Vizzini, Aiti
Format: Article
Language:English
Subjects:
Ciona robusta
Gene expression
Genes
Hemocytes
Hybridization
Inflammation
Inflammatory response
Leukocyte migration
Lipopolysaccharides
Macrophage migration inhibitory factor
Macrophages
Nucleotide sequence
Pharynx
Stimulation
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Summary:The effects of lipopolysaccharide (LPS) on Mif (macrophage migration inhibitory factor) gene expression in the pharynx (haemapoetic tissue) of Ciona robusta were investigated using quantitative reverse-transcription PCR (qRT-PCR) and in situ hybridisation (ISH). To verify the induction of an inflammatory response in the pharynx, a qRT-PCR analysis was performed to evaluate the change in the expression of proinflammatory marker genes such as Mbl, Ptx-like, Tnf-α and Nf-kb, which were shown to be upregulated 1 h post LPS challenge. The change in the expression of the two Mif paralogs in the pharynx was assessed before and after stimulation, and qRT-PCR and ISH results showed that, although Mif2 and Mif2 were expressed in clusters of haemocytes in pharynx vessels, only Mif1 expression increased after LPS stimulation. This indicates that the Mif genes are differently regulated and respond to different ambient inputs that need further analysis.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.15427