Role of pretreatment type and microbial mechanisms on enhancing volatile fatty acids production during anaerobic fermentation of refinery waste activated sludge

• Published in: Bioresource technology 2023-08, Vol.381, p.129122-129122, Article 129122
• Main Authors: Wang, Qinghong, Xin, Wenzhuo, Shao, Zhiguo, Usman, Muhammad, Li, Jin, Shang, Pengyin, Kou, Yue, Gamal El-Din, Mohamed, Chen, Chunmao
• Format: Article
• Language: English
• Subjects: Alkaline fermentation; Anaerobiosis; Carbohydrate Metabolism; Fatty Acids, Volatile; Fermentation; Glucans - metabolism; Hydrogen-Ion Concentration; Metabolic pathway; Microbial community; Refinery waste activated sludge composition; Sewage - microbiology; Volatile fatty acids production
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129122

•Alkaline, thermal, thermal-PMS and APG pretreatment methods were compared.•All pretreatments improved sludge hydrolysis and inhibited methanogenesis.•Alkaline addition showed best performance towards VFAs yield and sludge reduction.•Refractory substances released in thermal-PMS and APG pretreatments.•Firmicutes are significantly enriched and play an essential role for VFAs production. This study compared the effects of alkaline, thermal, thermal-peroxymonosulfate (PMS), and alkyl polyglucose (APG) pretreatments on volatile fatty acids (VFAs) production from refinery waste activated sludge (RWAS), including VFAs yield, composition, organics components, microbial communities, and the potential improvement of mechanisms. All pretreatments effectively enhanced the bioconversion of RWAS and consequently promoted the hydrolysis process, which inhibited the methanogenesis process. However, the release of lignin/carboxyl-rich alicyclic molecules (CRAM)-like compounds and tannin substances in Thermal-PMS and APG groups significantly influenced the acidogenesis and acetogenesis processes. Among all pretreatments, alkaline pretreatment showed the highest VFAs yield of 95.06 mg/g volatile solids (VS) and VS removal of 17%. This result could be associated with the enrichment of functional hydrolytic-acidification bacteria, such as Planococcus and Soehngenia, and increased metabolism of amino acids, carbohydrates, and nucleotides. By considering an economical and efficient perspective, this study recommended the alkaline pretreatment for the anaerobic fermentation of RWAS.