Biodegradable Microrobots for DNA Vaccine Delivery

The delivery of nucleic acid vaccine to stimulate host immune responses against Coronavirus disease 2019 shows promise. However, nucleic acid vaccines have drawbacks, including rapid clearance and poor cellular uptake, that limit their therapeutic potential. Microrobots can be engineered to sustain...

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Bibliographic Details
Published in:Advanced healthcare materials 2023-08, Vol.12 (21), p.e2202921-n/a
Main Authors: Chen, Shuxun, Tan, Zhiwu, Liao, Pan, Li, Yanfang, Qu, Yun, Zhang, Qi, Yang, Mingxuan, Chan, Kannie Wai Yan, Zhang, Li, Man, Kwan, Chen, Zhiwei, Sun, Dong
Format: Article
Language:English
Subjects:
Antigens
Biocompatibility
Biodegradability
biodegradable hydrogels
Coronaviruses
COVID-19
Dendritic cells
Deoxyribonucleic acid
DNA
DNA vaccines
Gelatin
Gene expression
Immune clearance
Immune system
Immunization
Microrobots
Microspheres
nanoparticles
Nucleic acids
Polyethyleneimine
vaccine delivery
Vaccines
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Summary:The delivery of nucleic acid vaccine to stimulate host immune responses against Coronavirus disease 2019 shows promise. However, nucleic acid vaccines have drawbacks, including rapid clearance and poor cellular uptake, that limit their therapeutic potential. Microrobots can be engineered to sustain vaccine release and further control the interactions with immune cells that are vital for robust vaccination. Here, the 3D fabrication of biocompatible and biodegradable microrobots via the two‐photon polymerization of gelatin methacryloyl (GelMA) and their proof‐of‐concept application for DNA vaccine delivery is reported. Programmed degradation and drug release by varying the local exposure dose in 3D laser lithography and further functionalized the GelMA microspheres with polyethyleneimine for DNA vaccine delivery to dendritic cell and primary cells is demonstrated. In mice, the DNA vaccine delivered by functionalized microspheres elicited fast, enhanced, and durable antigen expression, which may lead to prolonged protection. Furthermore, we demonstrate the maneuverability of microrobots by fabricating GelMA microspheres on magnetic skeletons. In conclusion, GelMA microrobots may provide an efficient vaccination strategy by controlling the expression duration of DNA vaccines. The development of biodegradable microrobots for DNA vaccine delivery by fabricating spherical structures through 3D laser lithography based on the two‐photon polymerization of gelatin methacryloyl (GelMA) is reported. Local cross‐linking level in the GelMA microstructure can be tailored by local exposure dose during 3D lithography, which confers multilayered GelMA microrobots with different degradable performances and sustained drug release.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.202202921