Biodegradable wound dressing-based collagen/hyaluronic acid loaded antibacterial agents for wound healing application

Three biodegradable wound dressing based on binary Collagen (COL), Hyaluronic acid (HA) crosslinked loaded with silver nanoparticles (AgNPs), Gentamicin (GENT) and AgNPs/GENT successfully prepared using freeze drying technique. Chemical evaluations for synthesized membranes were carried out using FT...

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Bibliographic Details
Published in:International journal of biological macromolecules 2023-07, Vol.242 (Pt 1), p.124700-124700, Article 124700
Main Authors: AlSalem, Huda S., Bukhari, Abeer Abdulaziz H.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Bandages
Collagen
Collagen - chemistry
Gentamicin
Gentamicins - pharmacology
Hyaluronic acid
Hyaluronic Acid - chemistry
Metal Nanoparticles
Saccharomyces cerevisiae - metabolism
Silver
Wound Healing
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Summary:Three biodegradable wound dressing based on binary Collagen (COL), Hyaluronic acid (HA) crosslinked loaded with silver nanoparticles (AgNPs), Gentamicin (GENT) and AgNPs/GENT successfully prepared using freeze drying technique. Chemical evaluations for synthesized membranes were carried out using FTIR- ATR. While physical properties were evaluated through swelling and degradation percent. Antibacterial activity was evaluated against G+, G-, yeast and fungi. Finally, cytotoxicity and wound healing evaluations were carried out against skin fibroblast normal cell line, while anti-inflammatory evaluated using RAW 264.7 macrophage cell line. The three produced membrane showed physically interaction between polymer network and the loaded antibiotic. Swelling properties showed superior results for three membranes. Degradability of prepared sheets was rapidly no more than three days. Toxicity evaluations and anti-inflammatory showed superior results for all examined samples except mixed with AgNPs and Gentamicin (GENT). Antibacterial activity showed resistance to G+, G- and yeast. All prepared sheet showed safe towards cell except COL/HA/AgNPs/GENT. Wound healing studied showed efficient of both COL/HA/AgNPs and COL/HA/GENT compared to blank and mixed membrane COL/HA/AgNPs/GENT. The obtained results recommended COL/HA loaded individually either AgNPs or Gentamicin (GENT) as antibacterial and wound healing sheet rather than mixed prepared membrane.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.124700