Study on Calceolarioside A Anti‐Aβ25–35‐Induced Damage in SH‐SY5Y cells by Atomic Force Microscopy

Calceolarioside A is a phenylethyl glycoside compound, originally isolated from the bark of Fraxinus mandshurica Rupr. In this work, the protective effect of Calceolarioside A on beta Amyloid protein induced toxicity in SH‐SY5Y cells was studied. DPPH experiment, MTT assay, SEM, Atomic force microsc...

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Bibliographic Details
Published in:Chemistry & biodiversity 2023-06, Vol.20 (6), p.e202300430-n/a
Main Authors: Chen, Yujuan, Qu, Kaige, Wang, Ying, Zhang, Qinghan, Xie, Ying, Wang, Zuobing
Format: Article
Language:English
Subjects:
antioxidants
Atomic force microscopy
Aβ25–35
calceolarioside A
Damage
Mechanical properties
Modulus of elasticity
SH-SY5Y cells
Survival
Toxicity
β-Amyloid
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Summary:Calceolarioside A is a phenylethyl glycoside compound, originally isolated from the bark of Fraxinus mandshurica Rupr. In this work, the protective effect of Calceolarioside A on beta Amyloid protein induced toxicity in SH‐SY5Y cells was studied. DPPH experiment, MTT assay, SEM, Atomic force microscopy and Colony formation were used to study the activity of Calceolarioside A. The results in this article show that the survival rate of the cells with Calceolarioside A (20–40 mg/mL) was significantly higher than that of the model cells without Calceolarioside A. Calceolarioside A could protect SH‐SY5Y cells by improving some parameters in cells, such as the cell height, Young's modulus, adhesion and branch. In summary, Calceolarioside A can reduce Aβ25–35‐induced damage in SH‐SY5Y cells. It can be a potential medicine to treatment with AD.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202300430