Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro

Aim of this study was to investigate the effects of the resin monomer BisGMA on the glutathione concentration (monobromobimane assay) and apoptosis (Annexin V/PI-assay) of cultured primary human gingival fibroblasts. Cells were treated for up to 24 h with 0.001–0.25 m m BisGMA to determine growth cu...

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Bibliographic Details
Published in:Biomaterials 2004-08, Vol.25 (19), p.4573-4580
Main Authors: Engelmann, Joern, Janke, Viktoria, Volk, Joachim, Leyhausen, Gabriele, Von Neuhoff, Nils, Schlegelberger, Brigitte, Geurtsen, Werner
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Biocompatible Materials - adverse effects
Biocompatible Materials - chemistry
BisGMA
Bisphenol A-Glycidyl Methacrylate - adverse effects
Bisphenol A-Glycidyl Methacrylate - chemistry
Cell Survival - drug effects
Cells, Cultured
Composite Resins - adverse effects
Composite Resins - chemistry
Dose-Response Relationship, Drug
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - metabolism
Gingiva - cytology
Gingiva - drug effects
Gingiva - metabolism
Gingival fibroblasts
Glutathione
Glutathione - metabolism
Humans
Materials Testing
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Summary:Aim of this study was to investigate the effects of the resin monomer BisGMA on the glutathione concentration (monobromobimane assay) and apoptosis (Annexin V/PI-assay) of cultured primary human gingival fibroblasts. Cells were treated for up to 24 h with 0.001–0.25 m m BisGMA to determine growth curves using the DNA stain H33342. Subsequent Annexin V/PI-assays revealed that fibroblasts exposed to concentrations of 0.005–0.01 m m (non-cytotoxic) and 0.05 m m (ED 10-concentration) showed no increase of the share of apoptotic cells compared to non-treated controls (5–8%), while 0.1 m m BisGMA (∼ ED 50-concentration) caused a significant increase of the percentage of apoptotic cells (50%). Simultaneously to the induction of apoptosis, 0.1 and 0.25 m m of BisGMA caused a significant depletion of the intracellular GSH content after 18 h of incubation. Our results indicate that BisGMA at concentrations >0.1 m m causes an extreme depletion of the intracellular GSH pool as well as apoptosis.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2003.11.048