Snail-inspired AFG/GelMA hydrogel accelerates diabetic wound healing via inflammatory cytokines suppression and macrophage polarization

Diabetic foot ulcers (DFUs) are a severe and rapidly growing diabetic complication, but treating DFUs remains a challenge for the existing therapies are expensive and highly non-responsive. Recently, we discovered that a natural adhesive from snail mucus can promote skin wound healing. Herein, inspi...

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Published in:Biomaterials 2023-08, Vol.299, p.122141-122141, Article 122141
Main Authors: Zhou, Zhipeng, Deng, Tuo, Tao, Maixian, Lin, Lisha, Sun, Luyun, Song, Xuemei, Gao, Dongxiu, Li, Jixing, Wang, Zhongjuan, Wang, Xingzi, Li, Jinpeng, Jiang, Zexiu, Luo, Lan, Yang, Lian, Wu, Mingyi
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials - pharmacology
Biomimetic hydrogels
Cytokines - metabolism
Diabetes Mellitus - metabolism
Diabetic wound healing
Gelatin - pharmacology
Glycosaminoglycan
Hydrogels - pharmacology
Inflammation suppression
Macrophage polarization
Macrophages - metabolism
Mice
Rats
Wound Healing
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Summary:Diabetic foot ulcers (DFUs) are a severe and rapidly growing diabetic complication, but treating DFUs remains a challenge for the existing therapies are expensive and highly non-responsive. Recently, we discovered that a natural adhesive from snail mucus can promote skin wound healing. Herein, inspired by the finding, we developed a double-network hydrogel biomaterial that composed of snail glycosaminoglycan (AFG) and methacrylated gelatin (GelMA), in which AFG is the main bioactive component of snail mucus and GelMA provides a scaffold mimicking the proteins in snail mucus. The biomimetic hydrogel exhibited strong tissue adhesion, potent anti-inflammatory activity, and excellent biocompatibility. The biodegradable AFG/GelMA hydrogel markedly promoted chronic wound healing in both STZ-induced type 1 diabetic rat and db/db mouse models after a single treatment. Further mechanistic research showed that the hydrogel significantly attenuated inflammation by sequestrating pro-inflammatory cytokines, as well as downregulated their expression by inhibiting NF-ĸB signaling pathway, and it can also promote macrophage polarization to M2 phenotype. Taken together, the bioinspired hydrogel can effectively promote the transition of chronic wounds from inflammation to proliferation stage. These data suggest that the AFG/GelMA hydrogel is a promising therapeutic biomaterial for the treatment of chronic diabetic wounds.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2023.122141