In Vitro Study of Cytostatic Activity of Baicalin, Baicalein, and Chlorophyllipt on HeLa-v Cell Line

Cytostatic activity of baicalin, baicalein, and neogalenical drug Chlorophyllipt was studied in vitro on HeLa-v cells. Standard samples of Eucalimin, baicalin, and baicalein, as well as Chlorophyllipt and paclitaxel (reference drug Taxacad) were used. The cell deaths were determined by MTT assay in...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2023-04, Vol.174 (6), p.738-740
Main Authors: Boyko, N. N., Novikov, O. O., Zhilyakova, E. T., Pisarev, D. I., Nadezhdin, S. V., Zubareva, E. V., Lysenko, A. S., Lapshina, E. G., Yagnikov, S. A., Nikitchenko, D. V.
Format: Article
Language:English
Subjects:
Baicalin
Biomedical and Life Sciences
Biomedicine
Cancer therapies
Cell Biology
Cell death
Chemotherapy
Cytostatic agents
Cytostatic Agents - pharmacology
Drugs
Flavanones - metabolism
Flavanones - pharmacology
Flavonoids
Flavonoids - pharmacology
HeLa Cells
Humans
Internal Medicine
Laboratory Medicine
Paclitaxel
Pathology
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Summary:Cytostatic activity of baicalin, baicalein, and neogalenical drug Chlorophyllipt was studied in vitro on HeLa-v cells. Standard samples of Eucalimin, baicalin, and baicalein, as well as Chlorophyllipt and paclitaxel (reference drug Taxacad) were used. The cell deaths were determined by MTT assay in a Multiskan FC microplate reader with incubator. The effective inhibition concentration (IC 50 ) of the tested substances were: paclitaxel (4.0±0.4 μM)—baicalein (10.5±1.1 μM)—baicalin (16.5±1.7 μM)—sum of euglobals in Chlorophyllipt (24.1±2.5 μM). Chlorophyllipt was found to exhibit cytostatic activity. Cytostatic activity of baicalein, baicalin, and Chlorophyllipt was lower than cytostatic activity of the reference drug by 2.6, 4.1, and 6 times, respectively. The prospects of further evaluation of the synergetic effect of baicalin, baicalein, and chlorophyllipt used in combinations with different cytostatic agents for finding the most effective combination have been shown.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05782-6