Evaluation of gene expression related to immunity, apoptosis, and gut integrity that underlies Artemisia's therapeutic effects in necrotic enteritis-challenged broilers

The experiment was designed to validate the effect of Artemisia annua and its novel commercial product (Navy Cox) on the control of necrotic enteritis (NE). A total of one hundred forty broiler chicks were randomly distributed into seven equal groups: G1, control negative; G2, infected with Eimeria...

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Bibliographic Details
Published in:3 Biotech 2023-06, Vol.13 (6), p.181-181, Article 181
Main Authors: El-Demerdash, Azza S., Mohamady, Sahar N., Megahed, Hend M., Ali, Naglaa M.
Format: Article
Language:English
Subjects:
Agriculture
Amoxicillin
Apoptosis
Artemisia annua
Bioinformatics
Biomaterials
Biotechnology
Blood
Cancer Research
Chemistry
Chemistry and Materials Science
Clostridium perfringens
Enteritis
Gene expression
Genes
Immune response (cell-mediated)
Immune system
Immunity
Immunology
Immunomodulation
Integrity
Interleukins
Intestine
Leukocytosis
Lysozyme
Monocytosis
Navy
Nitric oxide
Original Article
Stem Cells
Tight junctions
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Summary:The experiment was designed to validate the effect of Artemisia annua and its novel commercial product (Navy Cox) on the control of necrotic enteritis (NE). A total of one hundred forty broiler chicks were randomly distributed into seven equal groups: G1, control negative; G2, infected with Eimeria (day 15) and C. perfringens (day 19); G3, treated with Navy Cox before challenge; G4, treated with Artemisia before challenge; G5, infected and then treated with Navy Cox; G6, infected and then treated with Artemisia; and G7, infected and treated with amoxicillin. Chicken response and immune organ indicants were recorded during the observation period (4 weeks). Whole blood and serum samples were collected for immunological evaluation, and tissue samples were collected for bacterial counts and estimation of mRNA expression of genes encoding apoptosis, tight junctions, and immunity. Chickens in the infected group revealed a significant decrease in RBCS, HB, PCV% total protein, Lysozyme, and nitric oxide activity in addition to leukocytosis, heterophilia, monocytosis, increase in cortisol, interleukins, and malondialdehyde. Treated groups displayed lower lesions, colony-forming units, and no mortality. Concurrently, a complete blood profile, antioxidants, and immune markers showed significant improvements. The mRNA expression levels of CASP, CLDN-1, OCLN, TJPI, MUC2 , and cell-mediated immune response genes ( p  
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-023-03560-9