Indian traditional rice variety "Gathuwan" suppresses T-cell-mediated immune responses via activation of ERK/Nrf2/HO-1 signalling pathway

• Published in: Food & function 2023-06, Vol.14 (11), p.5232-5250
• Main Authors: Chauhan, Anjali, Checker, Rahul, Nair, Sunil, Sahu, Parmeshwar K, Sharma, Deepak, Das, Bikram K
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Cell activation; Cell death; Cell-free system; Cytokines; Cytokines - metabolism; Developing countries; Enrichment; Extracellular signal-regulated kinase; Food; Functional foods & nutraceuticals; Gene expression; Germplasm; Glutathione; Graft-versus-host reaction; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; High performance liquid chromatography; Hydroxybenzaldehydes; Hydroxycinnamic acid; Immune response (cell-mediated); Immunity; Immunomodulation; Indoles; Interleukin 2; Interleukin 4; Interleukin 6; Kinases; LDCs; Liquid chromatography; Lymphocytes; Lymphocytes T; MAP kinase; Mass spectrometry; Mass spectroscopy; Metabolic pathways; Metabolism; Metabolites; Mice; Morbidity; NF-E2-Related Factor 2 - metabolism; Nuclear transport; Oryza - metabolism; Oxidative Stress; Reactive oxygen species; Reactive Oxygen Species - metabolism; Rice; Scavenging; Signal transduction; Signaling; T-Lymphocytes - metabolism; Tandem Mass Spectrometry; Translocation; γ-Interferon
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo00125c

The impact of food on immune functions has been recognized for centuries and is now being increasingly explored for therapeutic applications. Rice, in addition to being the staple food in most developing countries, exhibits diverse complexities of phytochemicals among its wide germplasm repertoire, which supports its development as a functional food. In the present study, we have explored the immunomodulatory properties of Gathuwan rice, a local rice variety grown in Chhattisgarh, India, and traditionally used for the treatment of rheumatism. Methanolic Gathuwan Brown Rice Extract (BRE) inhibits T-cell activation and proliferation and cytokine secretion (IL-2, IL-4, IL-6 and IFN-γ) without inducing cell death. BRE exhibits radical scavenging activity in a cell-free system and decreases intracellular reactive oxygen species (ROS) and glutathione levels in lymphocytes. BRE induces nuclear translocation of the immune-regulatory transcription factor Nrf2 activation of ERK and p-38 MAP kinase and up-regulates the expression of Nrf2-dependent genes ( , , , and ) in lymphocytes. BRE treatment had no effect on cytokine secretion by lymphocytes from Nrf2 knockout mice, confirming the role of Nrf2 in the immunosuppressive effects of BRE. Feeding of Gathuwan brown rice to mice had no effect on the basal haematological parameters, but lymphocytes isolated from these mice were hypo-responsive to mitogenic stimuli. Treatment of allografts with BRE significantly prevented graft- -host disease (GVHD)-associated mortality and morbidity in mice. Metabolic pathway enrichment analysis of ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) data revealed a high enrichment ratio of amino acid and vitamin B metabolism pathways, and among metabolite sets, pyridoxamines, phytosphingosines, hydroxybenzaldehydes, hydroxycinnamic acids and indoles were highly enriched bioactive components. In conclusion, Gathuwan BRE suppresses T-cell-mediated immune responses by altering the cellular redox balance and activating the Nrf2 signalling pathway.