Dual-targeting exosomes for improved drug delivery in breast cancer

The authors investigated whether displaying more than one homing peptide enhanced the tumor-targeting efficiency of exosomes. Exosomes from human embryonic kidney cells (HEK293F) were engineered to display either mono- or dual-tumor-penetrating peptides, iRGD and tLyp1. Exosomes were purified via ta...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2023-03, Vol.18 (7), p.599-611
Main Authors: Tran, Nam Hb, Nguyen, Diem Dn, Nguyen, Ngoc Mai, Tran, Chau, Nguyen Thi, Ngoc Thanh, Ho, Duyen Tk, Nguyen, Hoai-Nghia, Tu, Lan N
Format: Article
Language:English
Subjects:
breast cancer
Breast Neoplasms - drug therapy
Cell Line, Tumor
doxorubicin
Doxorubicin - pharmacology
Drug Delivery Systems
Exosomes
Female
gene/drug delivery
Humans
iRGD
nanomedicine
Peptides
tLyp1
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Summary:The authors investigated whether displaying more than one homing peptide enhanced the tumor-targeting efficiency of exosomes. Exosomes from human embryonic kidney cells (HEK293F) were engineered to display either mono- or dual-tumor-penetrating peptides, iRGD and tLyp1. Exosomes were purified via tangential flow filtration followed by ultracentrifugation. When loaded with doxorubicin (Dox), the dual iRGD-tLyp1 exosomes strongly enhanced Dox uptake in both MCF-7 and MDA-MB-231 breast cancer cell lines, superior to single iRGD or tLyp1 exosomes. The dual iRGD-tLyp1 exosomal Dox was also the most potent, with IC /GI values being 3.7–17.0-times lower than those of free Dox and other exosomal Dox. Selecting appropriate combinatorial homing peptides could be an approach for future precision nanomedicine.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2022-0328