Evaluation of two fully automated ADAMTS13 activity assays in comparison to manual FRET assay

The objective of the present study was to evaluate and compare the validity and utility of two fully automated ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) activity assays for clinical diagnostic decision-making and to compare their performance. Two au...

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Bibliographic Details
Published in:International journal of laboratory hematology 2023-10, Vol.45 (5), p.758-765
Main Authors: Irsara, Christian, Anliker, Markus, Egger, Alexander E, Harasser, Lukas, Lhotta, Karl, Feistritzer, Clemens, Griesmacher, Andrea, Loacker, Lorin
Format: Article
Language:English
Subjects:
ADAM protein
Automation
Decision making
Patients
Remission
Statistical analysis
Stem cell transplantation
Thrombocytopenic purpura
Thrombospondin
Thrombotic microangiopathy
Thrombotic thrombocytopenic purpura
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Summary:The objective of the present study was to evaluate and compare the validity and utility of two fully automated ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13) activity assays for clinical diagnostic decision-making and to compare their performance. Two automated ADAMTS13 activity assays (Werfen HemosIL® AcuStar ADAMTS13 Activity, Technoclone Technofluor ADAMTS13 Activity) were compared with a manual FRET assay (BioMedica ACTIFLUOR ADAMTS13 Activity). The following samples were used: 13 acute phase TTP (thrombotic thrombocytopenic purpura) samples from 11 different patients, one sample from a patient with congenital ADAMTS13 deficiency, 16 samples from control patients, three follow-up samples from TTP patients in long-term remission and one sample from a patient with stem cell transplantation related thrombotic microangiopathy (TMA). The WHO 1st International Standard for ADAMTS13 and several dilutions of normal plasma with ADAMTS13-depleted normal plasma were also tested. Statistical analysis included descriptive statistics, sensitivity and specificity, Passing & Bablok regression and Bland-Altman plot. The quantitative comparison between the HemosIL® (x) and Technofluor (y) methods showed a strong correlation (Pearson r = 0.98, n = 49). When considering an ADAMTS13 activity of
ISSN:1751-5521
1751-553X
1751-553X
DOI:10.1111/ijlh.14090