Facile modification of nanochitin in aqueous media for stabilizing tea tree oil based Pickering emulsion with prolonged antibacterial performance

• Published in: International journal of biological macromolecules 2023-07, Vol.242 (Pt 3), p.124873-124873, Article 124873
• Main Authors: Li, Xinxia, Liu, Ying, Chen, Feier, Liu, Liang, Fan, Yimin
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Chitin nanofibril; Emulsions - chemistry; Escherichia coli; Essential oil; Particle Size; Pickering emulsion; Schiff base; Tea Tree Oil; Zwitterionic
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.124873

Nanochitins have been explored for preparing Pickering Emulsions, however its application is restricted by its simplex disperse nature. It was hypothesized that zwitterionic nanochitins should be capable of stabilizing oil/water (O/W) interfaces in wider pH range. Furthermore, the control of their size, disperse nature and self-assembly performance suggest the formulation of tunable emulsions. Zwitterionic nanochitins were prepared via Schiff base reaction. A systematic study was performed analyzing the disperse nature, fibril morphology, surface characteristic of modified nanochitins. Oil-in-Water Pickering Emulsions stabilized by modified nanochitins were formulated and emulsion stability was analyzed as function of concentration, pH and self-assembly property and further applied for prolonged antibacterial applications. Comparing freshly prepared nanochitins, neutral/alkaline stably dispersed nanochitins can be prepared while maintaining fibril characteristics such as fibril size, crystallinity, thermal stability and so on. Better suspension stability of modified nanochitins under alkaline conditon together with the self assembly performance resulting from amino groups and carboxyl groups benefit the enhanced emulsion stability under nanochitins concentreation of 0.2 %. Encapsulation of tea tree oil in Pickering Emulsions prolongs the diffusion rate oil in the aqueous environment, thus resulting prolongs its antibacterial performance against E. coli and B. subtilis. [Display omitted] •Dispersibilty of nanochitin can be adjusted by grafting glyoxylic acid.•Stable Pickering emulsion can be prepared using modified nanochitins.•Self-assembly between modified nanochitins improves emulsion stability.•Emulsion encapsulated tea tree oil possess prolonged antibacterial activity.