Connections of the sheep basolateral amygdala: A diffusion tensor imaging study

• Published in: Journal of neuroscience methods 2023-06, Vol.393, p.109883-109883, Article 109883
• Main Authors: Graïc, J.M., Tagliavia, C., Salamanca, G., Gerussi, T., Grandis, A., Cozzi, B., Bombardi, C.
• Format: Article
• Language: English
• Subjects: Amygdala; Amygdala - physiology; Animals; Basolateral complex; Basolateral Nuclear Complex; Cerebral Cortex; Connections; Diffusion Tensor Imaging; Mammals; Neural Pathways; Rats; Sheep; Tractography
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/j.jneumeth.2023.109883

A large amount of anatomic data published over the past decade has provided novel insight into the connections of the basolateral amygdala (BLA) in the rat, cat, and monkey. The mammalian (rat, cat, and monkey) BLA has strong connections with the cortex (especially piriform, and frontal cortices), the hippocampal region (especially perirhinal cortex, entorhinal cortex, and subiculum), the thalamus (in particular, the posterior internuclear nucleus and medial geniculate nucleus) and, to some extent, the hypothalamus. An important question remains as to how well the data obtained in rodents and primates can be extrapolated to ruminants. To address this issue the connections of the sheep BLA has been determined by Magnetic Resonance Imaging (MRI) and Diffusion Tensor Imaging (DTI, Tractography). Tractography showed ipsilateral connections between the BLA and several areas. Reviews based mainly on description of the results obtained using anterograde and retrograde neuronal tracers. In the present research, we prefer to use a non-invasive technique (DTI). This report shows the existence of specific amygdaloid connections in the sheep. •Structural imaging analysis by MRI and DTI can be used for tractography.•Sheep is an interesting mammalian species in comparative neuroscience studies.•The sheep basolateral amygdala relates to a wide variety of brain areas.