Preparation of a shared chip for cell capturing and imaging with nanoparticle scaffold, nanogap and circulating tumor cell detection

To achieve accurate detection of circulating tumor cells (CTCs) in a large-volume sample. Silica nanoparticles were crosslinked layer-by-layer on glass slides as the substrate of a chip using polyacrylic acid. Polyacrylic acid was immobilized as a spacer and capture ligands were immobilized on the s...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2023-04, Vol.18 (8), p.667-678
Main Authors: Lu, Yin-Qiu, Zeng, Qi, He, Huan-Huan, Zhang, Jing-Shuo, Xie, Hong-Ping
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Separation
CTC
detected cell number
Diagnostic Imaging
Humans
nano-gap
nanoparticle-scaffold
Nanoparticles
Neoplastic Cells, Circulating
shared chip
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Summary:To achieve accurate detection of circulating tumor cells (CTCs) in a large-volume sample. Silica nanoparticles were crosslinked layer-by-layer on glass slides as the substrate of a chip using polyacrylic acid. Polyacrylic acid was immobilized as a spacer and capture ligands were immobilized on the spacer. The chip can be integrally applied to capture, post-treatment and imaging detection for CTCs. The detected cell numbers were 33 and 40 for 9 cell/ml samples and clinical blood samples (7.5 ml), respectively. The detection ratio of positive samples was 100%. The significantly increased detected number for CTCs indicates that this methodology may avoid or greatly reduce the false-negative ratio of positive clinical samples.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2022-0303