Novel delivery of sorafenib by natural killer cell-derived exosomes-enhanced apoptosis in triple-negative breast cancer

We investigated the delivery of sorafenib (SFB) to breast cancer spheroids by natural killer cell-derived exosomes (NK-Exos). SFB-NK-Exos were constructed by electroporation. Their antitumor effects were evaluated by methyl thiazolyl tetrazolium, acridine orange/ethidium bromide, 4′,6-diamidino-2-ph...

Full description

Saved in:
Bibliographic Details
Published in:Nanomedicine (London, England) England), 2023-02, Vol.18 (5), p.437-453
Main Authors: Hashemi, Zahra Sadat, Ghavami, Mahlegha, Kiaie, Seyed Hossein, Mohammadi, Fateme, Barough, Mahdieh Shokrollahi, Khalili, Saeed, Hosseini-Farjam, Zahra, Mossahebi-Mohammadi, Majid, Sheidary, Alireza, Ghavamzadeh, Ardeshir, Forooshani, Ramin Sarrami
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
breast cancer
Cell Line, Tumor
cytotoxic effect
drug release
exosome
Exosomes
Humans
Killer Cells, Natural
nanocarrier
natural killer cell
sorafenib
Sorafenib - pharmacology
spheroid
Triple Negative Breast Neoplasms - drug therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We investigated the delivery of sorafenib (SFB) to breast cancer spheroids by natural killer cell-derived exosomes (NK-Exos). SFB-NK-Exos were constructed by electroporation. Their antitumor effects were evaluated by methyl thiazolyl tetrazolium, acridine orange/ethidium bromide, 4′,6-diamidino-2-phenylindole, annexin/propidium iodide, scratch and migration assay, colony formation, RT-PCR, western blot and lipophagy tests. The loading efficacy was 46.66%. SFB-NK-Exos-treated spheroids showed higher cytotoxic effects (33%) and apoptotic population (44.9%). Despite the reduction of SFB concentration in the SFB-NK-Exos formulation, similar cytotoxic effects to those of free SFB were observed. Increased intracellular trafficking, sustained release of the drug and selective inhibitory effects demonstrated efficient navigation. This is the first report for SFB loading into NK-Exos, which led to significant cytotoxic intensification against cancer cells.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2022-0237