Response mechanism of lettuce (Lactuca sativa L.) under combined stress of Cd and DBDPE: An integrated physiological and metabolomics analysis

• Published in: The Science of the total environment 2023-08, Vol.887, p.164204-164204, Article 164204
• Main Authors: Qiao, Zhihua, Luo, Kailun, Zhou, Shanqi, Fu, Mengru, Shao, Xuechun, Gong, Kailin, Peng, Cheng, Zhang, Wei
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Brominated flame retardants; Cadmium - metabolism; Heavy metal; Lactuca - metabolism; Lettuce; Metabolomics; Toxicology; Vegetables - metabolism
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.164204

DBDPE and Cd are representative contaminants commonly found in electronic waste (e-waste), which tend to be gradually discharged and accumulated in the environment during e-waste dismantling, resulting in frequent outbreaks and detection of these pollutants. The toxicity of both chemicals to vegetables after combined exposure has not been determined. The accumulation and mechanisms of phytotoxicity of the two compounds, alone and in combination, were studied using lettuce. The results showed that the enrichment ability of Cd and DBDPE in root was significantly higher than that in aerial part. Exposure to 1 mg/L Cd + DBDPE reduced the toxicity of Cd to lettuce, while exposure to 5 mg/L Cd + DBDPE increased the toxicity of Cd to lettuce. The absorption of Cd in the underground part of lettuce of 5 mg/L Cd + DBDPE was significantly increased by 108.75 % compared to 5 mg/L Cd. The significant enhancement of antioxidant system activity in lettuce under 5 mg/L Cd + DBDPE exposure, and the root activity and total chlorophyll content were decreased by 19.62 % and 33.13 %, respectively, compared to the control. At the same time, the organelles and cell membranes of lettuce root and leaf were significantly damaged, which was significantly worse than that of single Cd and DBDPE treatment. Combined exposure significantly affected the pathways related to amino acid metabolism, carbon metabolism and ABC transport in lettuce. This study filled the safety gap of DBDPE and Cd combined exposure on vegetables and would provide a theoretical basis for the environmental behavior and toxicological study of DBDPE and Cd. [Display omitted] •DBDPE can reduce the uptake of 1 mg/L Cd and promote the uptake of 5 mg/L Cd in lettuce.•Combined exposure damaged the organelles of lettuce, resulting in plasmolysis.•Cd and DBDPE caused downregulation of the amino acid metabolism pathway of lettuce.