Y‐box binding protein 1 influences zygotic genome activation by regulating N6‐methyladenosine in porcine embryos

Y‐box binding protein 1 (YBX1) is a member of the family of DNA‐ and RNA‐binding proteins that play crucial roles in multiple aspects, including RNA stabilization, translational repression, and transcriptional regulation; however, its roles in embryo development remain less known. In this study, to...

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Bibliographic Details
Published in:Journal of cellular physiology 2023-07, Vol.238 (7), p.1592-1604
Main Authors: Jiang, Wen‐Jie, Sun, Ming‐Hong, Li, Xiao‐Han, Lee, Song‐Hee, Heo, Geun, Zhou, Dongjie, Cui, Xiang‐Shun
Format: Article
Language:English
Subjects:
Adenosine
Adenosine - metabolism
Animals
Blastocysts
Cytoplasm
DNA-Binding Proteins - metabolism
embryo development
Embryogenesis
Embryonic Development
Embryonic growth stage
Embryos
Gene expression
Gene regulation
Gene silencing
Genomes
Growth factors
Histones
m6A
Methyltransferase
N6-methyladenosine
porcine
Proteins
Ribonucleic acid
RNA
RNA modification
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-binding protein
siRNA
Swine
YBX1
ZGA
Zygote - metabolism
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Summary:Y‐box binding protein 1 (YBX1) is a member of the family of DNA‐ and RNA‐binding proteins that play crucial roles in multiple aspects, including RNA stabilization, translational repression, and transcriptional regulation; however, its roles in embryo development remain less known. In this study, to investigate the function of YBX1 and its mechanism of action in porcine embryo development, YBX1 was knocked down by microinjecting YBX1 siRNA at the one‐cell stage. YBX1 is located in the cytoplasm during embryonic development. The mRNA level of YBX1 was increased from the four‐cell stage to the blastocyst stage but was significantly decreased in YBX1 knockdown embryos compared with the control. Moreover, the percentage of blastocysts was decreased following YBX1 knockdown compared with the control. Defecting YBX1 expression increased maternal gene mRNA expression and decreased zygotic genome activation (ZGA) gene mRNA expression and histone modification owing to decreased levels of N6‐methyladenosine (m6A) writer N6‐adenosine‐methyltransferase 70 kDa subunit (METTL3) and reader insulin‐like growth factor 2 mRNA‐binding protein (IGF2BP1). In addition, IGF2BP1 knockdown showed that YBX1 regulated the ZGA process through m6A modification. In conclusion, YBX1 is essential for early embryo development because it regulates the ZGA process. Schematic figure depicting the role of YBX1 in pig embryonic development. YBX1 is essential for embryo development. YBX1 knockdown further affect ZGA by increasing maternal genes expression and decreasing ZGA genes expression. YBX1 knockdown further regulated histone modification, affecting gene activation. YBX1 regulated ZGA process by modulating m6A modification.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.31040