A portable electrochemical immunosensor for ovarian cancer uses hierarchical microporous carbon material from waste coffee grounds

A simple label-free electrochemical immunosensor for ovarian cancer (OC) detection was developed using a hierarchical microporous carbon material fabricated from waste coffee grounds (WCG). The analysis method exploited near-field communication (NFC) and a smartphone-based potentiostat. Waste coffee...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-06, Vol.190 (6), p.232-232, Article 232
Main Authors: Cotchim, Suparat, Thavarungkul, Panote, Kanatharana, Proespichaya, Thantipwan, Thaweesak, Jiraseree-amornkun, Amorn, Wannapob, Rodtichoti, Limbut, Warakorn
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Antibodies
Antigens
Biosensing Techniques - methods
Cancer
Carbon
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Coffee
Correlation coefficients
Electrochemical impedance spectroscopy
Electrochemical Techniques - methods
Electrodes
Female
Gold
Gold - chemistry
Humans
Hydroxides
Immunoassay - methods
Immunosensors
Metal Nanoparticles - chemistry
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Near field communication
Original Paper
Ovarian cancer
Ovarian Neoplasms - diagnosis
Potassium hydroxides
Sensors
Smart phones
Viral antibodies
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Summary:A simple label-free electrochemical immunosensor for ovarian cancer (OC) detection was developed using a hierarchical microporous carbon material fabricated from waste coffee grounds (WCG). The analysis method exploited near-field communication (NFC) and a smartphone-based potentiostat. Waste coffee grounds were pyrolyzed with potassium hydroxide and used to modify a screen-printed electrode. The modified screen-printed electrode was decorated with gold nanoparticles (AuNPs) to capture a specific antibody. The modification and immobilization processes were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor had an effective dynamic range of 0.5 to 50.0 U mL -1 of cancer antigen 125 (CA125) tumor marker with a correlation coefficient of 0.9995. The limit of detection (LOD) was 0.4 U mL -1 . A comparison of the results obtained from human serum analysis with the proposed immunosensor and the results obtained from the clinical method confirmed the accuracy and precision of the proposed immunosensor. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-05798-9