Microbial Reductive Dechlorination by a Commercially Available Dechlorinating Consortium Is Not Inhibited by Perfluoroalkyl Acids (PFAAs) at Field-Relevant Concentrations

• Published in: Environmental science & technology 2023-06, Vol.57 (22), p.8301-8312
• Main Authors: Hnatko, Jason P., Liu, Chen, Elsey, Jack L., Dong, Sheng, Fortner, John D., Pennell, Kurt D., Abriola, Linda M., Cápiro, Natalie L.
• Format: Article
• Language: English
• Subjects: Batch reactors; Biodegradation; Biodegradation, Environmental; Bioremediation; Chlorination; Chloroflexi - genetics; Chloroflexi - metabolism; Dechlorination; Ethene; Ethylene; Ethylenes - metabolism; Fluorocarbons; Mathematical models; Microcosms; Microorganisms; Numerical models; Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants; Perfluoroalkyl & polyfluoroalkyl substances; Perfluorochemicals; Plumes; Reactors; Respiration; Soils; Substrates; Trichloroethylene - metabolism; Vinyl chloride; Vinyl Chloride - metabolism
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.2c04815

Perfluoroalkyl acids (PFAAs) have been shown to inhibit biodegradation (i.e., organohalide respiration) of chlorinated ethenes. The potential negative impacts of PFAAs on microbial species performing organohalide respiration, particularly Dehalococcoides mccartyi (Dhc), and the efficacy of in situ bioremediation are a critical concern for comingled PFAA-chlorinated ethene plumes. Batch reactor (no soil) and microcosm (with soil) experiments, containing a PFAA mixture and bioaugmented with KB-1, were completed to assess the impact of PFAAs on chlorinated ethene organohalide respiration. In batch reactors, PFAAs delayed complete biodegradation of cis-1,2-dichloroethene (cis-DCE) to ethene. Maximum substrate utilization rates (a metric for quantifying biodegradation rates) were fit to batch reactor experiments using a numerical model that accounted for chlorinated ethene losses to septa. Fitted values for cis-DCE and vinyl chloride biodegradation were significantly lower (p < 0.05) in batch reactors containing ≥50 mg/L PFAAs. Examination of reductive dehalogenase genes implicated in ethene formation revealed a PFAA-associated change in the Dhc community from cells harboring the vcrA gene to those harboring the bvcA gene. Organohalide respiration of chlorinated ethenes was not impaired in microcosm experiments with PFAA concentrations of 38.7 mg/L and less, suggesting that a microbial community containing multiple strains of Dhc is unlikely to be inhibited by PFAAs at lower, environmentally relevant concentrations.