Detection of Treatment Response in Triple‐Negative Breast Tumors to Paclitaxel Using MRI Cell Size Imaging

Background Breast cancer treatment response evaluation using the response evaluation criteria in solid tumors (RECIST) guidelines, based on tumor volume changes, has limitations, prompting interest in novel imaging markers for accurate therapeutic effect determination. Purpose To use MRI‐measured ce...

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Bibliographic Details
Published in:Journal of magnetic resonance imaging 2024-02, Vol.59 (2), p.575-584
Main Authors: Jiang, Xiaoyu, McKinley, Eliot T., Xie, Jingping, Gore, John C., Xu, Junzhong
Format: Article
Language:English
Subjects:
Animal models
Apoptosis
Biomarkers
Breast cancer
Cancer therapies
Cell cycle
Cell size
Chemotherapy
diffusion time
Dimethyl sulfoxide
Evaluation
Field strength
Histology
In vivo methods and tests
Light microscopy
Limbs
Magnetic resonance imaging
Mathematical models
Medical imaging
Microscopy
microstructure imaging
Model testing
Optical microscopy
oscillating gradient
Paclitaxel
Parameters
Pellets
Population studies
Size distribution
Solid tumors
Statistical analysis
Statistical tests
treatment response
Tumors
Variance analysis
Xenotransplantation
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Summary:Background Breast cancer treatment response evaluation using the response evaluation criteria in solid tumors (RECIST) guidelines, based on tumor volume changes, has limitations, prompting interest in novel imaging markers for accurate therapeutic effect determination. Purpose To use MRI‐measured cell size as a new imaging biomarker for assessing chemotherapy response in breast cancer. Study Type Longitudinal; animal model. Study Population Triple‐negative human breast cancer cell (MDA‐MB‐231) pellets (4 groups, n = 7) treated with dimethyl sulfoxide (DMSO) or 10 nM of paclitaxel for 24, 48, and 96 hours, and 29 mice with MDA‐MB‐231 tumors in right hind limbs treated with paclitaxel (n = 16) or DMSO (n = 13) twice weekly for 3 weeks. Field Strength/Sequence Oscillating gradient spin echo and pulsed gradient spin echo sequences at 4.7 T. Assessment MDA‐MB‐231 cells were analyzed using flowcytometry and light microscopy to assess cell cycle phases and cell size distribution. MDA‐MB‐231 cell pellets were MR imaged. Mice were imaged weekly, with 9, 6, and 14 being sacrificed for histology after MRI at weeks 1, 2, and 3, respectively. Microstructural parameters of tumors/cell pellets were derived by fitting diffusion MRI data to a biophysical model. Statistical Tests One‐way ANOVA compared cell sizes and MR‐derived parameters between treated and control samples. Repeated measures 2‐way ANOVA with Bonferroni post‐tests compared temporal changes in MR‐derived parameters. A P‐value
ISSN:1053-1807
1522-2586
1522-2586
DOI:10.1002/jmri.28774