Bioremediation of aniline aerofloat wastewater at extreme conditions using a novel isolate Burkholderia sp. WX-6 immobilized on biochar

• Published in: Journal of hazardous materials 2023-08, Vol.456, p.131668-131668, Article 131668
• Main Authors: Ye, Jin-Cheng, Zhao, Qiu-Shi, Liang, Jin-Wei, Wang, Xiao-Xiao, Zhan, Zhen-Xuan, Du, Huan, Cheng, Ji-Liang, Xiang, Lei, Feng, Nai-Xian, Liu, Bai-Lin, Li, Yan-Wen, Li, Hui, Cai, Quan-Ying, Zhao, Hai-Ming, Mo, Ce-Hui
• Format: Article
• Language: English
• Subjects: Aniline aerofloat; Biochar-immobilization; Burkholderia sp; Flotation wastewater; Water bioremediation
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2023.131668

Aniline aerofloat (AAF) is a refractory organic pollutant in floatation wastewater. Little information is currently available on its biodegradation. In this study, a novel AAF-degrading strain named Burkholderia sp. WX-6 was isolated from mining sludge. The strain could degrade more than 80% of AAF at different initial concentrations (100–1000 mg/L) within 72 h. AAF degrading curves were fitted well with the four-parameter logistic model (R2 >0.97), with the degrading half-life ranging from 16.39 to 35.55 h. This strain harbors metabolic pathway for complete degradation of AAF and is resistant to salt, alkali, and heavy metals. Immobilization of the strain on biochar enhanced both tolerance to extreme conditions and AAF removal, with up to 88% of AAF removal rate in simulated wastewater under alkaline (pH 9.5) or heavy metal pollution condition. In addition, the biochar-immobilized bacteria removed 59.4% of COD in the wastewater containing AAF and mixed metal ions within 144 h, significantly (P