Subcutaneous mast cell tumours: A prospective multi‐institutional clinicopathological and prognostic study of 43 dogs

• Published in: Veterinary record 2023-07, Vol.193 (1), p.no-no
• Main Authors: Marconato, Laura, Stefanello, Damiano, Solari Basano, Fabrizio, Faroni, Eugenio, Dacasto, Mauro, Giantin, Mery, Bettini, Giuliano, Aresu, Luca, Bonfanti, Ugo, Bertazzolo, Walter, Annoni, Maurizio, Lecchi, Cristina, Sabattini, Silvia
• Format: Article
• Language: English
• Subjects: Animals; canine; cytograding; Dog Diseases - diagnosis; Dog Diseases - genetics; Dog Diseases - therapy; Dogs; lymph node metastasis; Lymph Nodes - pathology; Mast Cells - metabolism; Mast Cells - pathology; mastocytoma; Medical prognosis; Metastasis; Prognosis; Prospective Studies; Tumors; Veterinary medicine; Vinblastine
• ISSN: 0042-4900; 2042-7670
• DOI: 10.1002/vetr.2991

Background Canine subcutaneous mast cell tumours (ScMCTs) reportedly have a good prognosis. However, biomarkers that can be used to predict outcome are currently limited. Methods A multicentre prospective study was conducted to identify new prognostic markers. Dogs with a first occurrence of ScMCT were enrolled upon primary tumour removal and regional lymphadenectomy. In the absence of metastasis, dogs were monitored, while dogs with overtly metastatic lymph nodes (histological node 3, HN3) received adjuvant vinblastine. Results Forty‐three dogs were enrolled: 15 (34.9%) had at least one HN3 lymph node and received vinblastine, and 28 (65.1%) were monitored. Three tumours harboured exon 8 and 9 c‐kit mutations. Eight (18.6%) dogs experienced tumour progression, and five (11.6%) died of MCT‐related causes. The 1‐ and 2‐year survival rates were 90% and 77%, respectively. Variables significantly associated with an increased risk of progression included high cytograde, a mitotic count (MC) greater than 4/10 high‐power fields (hpf) and Ki67‐index greater than 23. An MC greater than 4/10 hpf was also associated with an increased risk of tumour‐related death. Limitations Regional rather than sentinel lymphadenectomy was performed in these dogs. Dogs were enrolled in oncology referral centres, constituting a different population compared to previous studies. Conclusions ScMCTs have a good prognosis. However, the metastatic rate at admission was higher in this study than previously reported, and a subset of tumours were associated with a fatal outcome despite multimodal treatment. Proliferative activity and cytograding may predict more aggressive behaviour in ScMCTs.