ATP‐Responsive Nanoparticles Covered with Biomolecular Machine “Chaperonin GroEL”

Herein, we report an ATP‐responsive nanoparticle (GroELNP) whose surface is fully covered with the biomolecular machine “chaperonin protein GroEL”. GroELNP was synthesized by DNA hybridization between a gold NP with DNA strands on its surface and GroEL carrying complementary DNA strands at its apica...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-08, Vol.62 (31), p.e202304894-n/a
Main Authors: Shen, Hao K., Morishita, Kiyoshi, Hashim, P. K., Okuro, Kou, Kashiwagi, Daiki, Kimura, Ayumi, Yanagisawa, Haruaki, Kikkawa, Masahide, Niwa, Tatsuya, Taguchi, Hideki, Aida, Takuzo
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
ATP
Biomolecular Machine
Chaperonin 60 - chemistry
Chaperonins - metabolism
Deoxyribonucleic acid
DNA
Fluorescence
Green fluorescent protein
GroEL
Host–Guest Chemistry
Hybridization
Nanoparticle
Nanoparticles
Protein Assembly
Protein Folding
Proteins
Strands
Transmission electron microscopy
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Summary:Herein, we report an ATP‐responsive nanoparticle (GroELNP) whose surface is fully covered with the biomolecular machine “chaperonin protein GroEL”. GroELNP was synthesized by DNA hybridization between a gold NP with DNA strands on its surface and GroEL carrying complementary DNA strands at its apical domains. The unique structure of GroELNP was visualized by transmission electron microscopy including under cryogenic conditions. The immobilized GroEL units retain their machine‐like function and enable GroELNP to capture denatured green fluorescent protein and release it in response to ATP. Interestingly, the ATPase activity of GroELNP per GroEL was 4.8 and 4.0 times greater than those of precursor cysGroEL and its DNA‐functionalized analogue, respectively. Finally, we confirmed that GroELNP could be iteratively extended to double‐layered (GroEL)2 ${{^{({\rm GroEL}){_{2}}}$ NP. A gold nanoparticle covered with the biomolecular machine “chaperonin GroEL” (GroELNP) allows the capture of denatured proteins and their controlled release in response to ATP and shows a nearly 5‐times enhanced ATPase activity compared to the precursor GroEL. Building on GroELNP, (GroEL)2 ${{^{({\rm GroEL}){_{2}}}$ NP having two layers of GroEL was iteratively synthesized, demonstrating the potential to construct higher‐order structures therefrom.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202304894